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  1. Artikel: Plasmid DNA encapsulation within cationic diblock copolymer vesicles for gene delivery.

    Korobko, A V / Backendorf, C / van der Maarel, J R C

    The journal of physical chemistry. B

    2006  Band 110, Heft 30, Seite(n) 14550–14556

    Abstract: We report the design and structural characterization of cationic diblock copolymer vesicles loaded with plasmid DNA based on a single emulsion technique. For this purpose, a DNA solution was emulsified in an organic solvent and stabilized by an ... ...

    Abstract We report the design and structural characterization of cationic diblock copolymer vesicles loaded with plasmid DNA based on a single emulsion technique. For this purpose, a DNA solution was emulsified in an organic solvent and stabilized by an amphiphilic diblock copolymer. The neutral block forms an interfacial brush, whereas the cationic attachment complexes with DNA. A subsequent change of the quality of the organic solvent results in the collapse of the brush and the formation of a capsule. The capsules are subsequently dispersed in aqueous medium to form vesicles and stabilized with an osmotic agent in the external phase. Inside the vesicles, the plasmid is compacted in a liquid-crystalline fashion as shown by the appearance of birefringent textures under crossed polarizers and the increase in fluorescence intensity of labeled DNA. The compaction efficiency and the size distribution of the vesicles were determined by light and electron microscopy, and the integrity of the DNA after encapsulation and subsequent release was confirmed by gel electrophoresis. We demonstrate reverse transfection of in vitro cultured HeLa cancer cells growing on plasmid-copolymer vesicles deposited on a glass substrate.
    Mesh-Begriff(e) Cations ; Cell Membrane Permeability ; DNA/administration & dosage ; DNA/chemistry ; DNA/genetics ; Drug Stability ; Emulsions ; Gene Transfer Techniques ; Green Fluorescent Proteins/genetics ; HeLa Cells ; Humans ; Microscopy, Electron, Scanning ; Microscopy, Phase-Contrast ; Microscopy, Polarization ; Plasmids/administration & dosage ; Plasmids/chemistry ; Plasmids/genetics ; Polyvinyls/chemistry ; Transfection
    Chemische Substanzen Cations ; Emulsions ; Polyvinyls ; enhanced green fluorescent protein ; poly(butadiene-b-N-methyl-4-vinylpyridinium iodide) ; Green Fluorescent Proteins (147336-22-9) ; DNA (9007-49-2)
    Sprache Englisch
    Erscheinungsdatum 2006-08-03
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1520-6106
    ISSN 1520-6106
    DOI 10.1021/jp057363b
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel: Encapsulation of DNA by cationic diblock copolymer vesicles.

    Korobko, A V / Jesse, W / van der Maarel, J R C

    Langmuir : the ACS journal of surfaces and colloids

    2005  Band 21, Heft 1, Seite(n) 34–42

    Abstract: Encapsulation of dsDNA fragments (contour length 54 nm) by the cationic diblock copolymer poly(butadiene-b-N-methyl-4-vinyl pyridinium) [PBd-b-P4VPQ] has been studied with phase contrast, polarized light, and fluorescence microscopies, as well as ... ...

    Abstract Encapsulation of dsDNA fragments (contour length 54 nm) by the cationic diblock copolymer poly(butadiene-b-N-methyl-4-vinyl pyridinium) [PBd-b-P4VPQ] has been studied with phase contrast, polarized light, and fluorescence microscopies, as well as scanning electron microscopy. Encapsulation was achieved with a single emulsion technique. For this purpose, an aqueous DNA solution is emulsified in an organic solvent (toluene) and stabilized by the amphiphilic diblock copolymer. The PBd block forms an interfacial brush, whereas the cationic P4VPQ block complexes with DNA. A subsequent change of the quality of the organic solvent results in a collapse of the PBd brush and the formation of a capsule. Inside the capsules, the DNA is compacted as shown by the appearance of birefringent textures under crossed polarizers and the increase in fluorescence intensity of labeled DNA. The capsules can also be dispersed in an aqueous medium to form vesicles, provided they are stabilized with an osmotic agent [poly(ethylene glycol)] in the external phase. It is shown that the DNA is released from the vesicles once the osmotic pressure drops below 10(5) N/m(2) or if the ionic strength of the supporting medium exceeds 0.1 M. The method has also proven to be efficient to encapsulate pUC18 plasmid in submicrometer-sized vesicles, and the general applicability of the method has been demonstrated by the preparation of the charge inverse system: cationic poly(ethylene imine) encapsulated by the anionic diblock poly(styrene-b-acrylic acid).
    Sprache Englisch
    Erscheinungsdatum 2005-01-04
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 2005937-1
    ISSN 1520-5827 ; 0743-7463
    ISSN (online) 1520-5827
    ISSN 0743-7463
    DOI 10.1021/la047967r
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel: Structure of strongly interacting polyelectrolyte diblock copolymer micelles.

    Korobko, A V / Jesse, W / Lapp, A / Egelhaaf, S U / van der Maarel, J R C

    The Journal of chemical physics

    2005  Band 122, Heft 2, Seite(n) 24902

    Abstract: The structure of spherical micelles of the diblock poly(styrene-block-acrylic acid) [PS-b-PA] copolymer in water was investigated up to concentrations where the polyelectrolyte coronal layers have to shrink and/or interpenetrate in order to accommodate ... ...

    Abstract The structure of spherical micelles of the diblock poly(styrene-block-acrylic acid) [PS-b-PA] copolymer in water was investigated up to concentrations where the polyelectrolyte coronal layers have to shrink and/or interpenetrate in order to accommodate the micelles in the increasingly crowded volume. We obtained the partial structure factors pertaining to the core and corona density correlations with small angle neutron scattering and contrast matching in the water. The counterion structure factor was obtained with small angle x-ray scattering (SAXS) with a synchrotron radiation source. Furthermore, we have measured the flow curves and dynamic visco-elastic moduli. The functionality of the micelles is fixed with a 9 nm diameter PS core and a corona formed by around 100 PA arms. As shown by the SAXS intensities, the counterions are distributed in the coronal layer with the same density profile as the corona forming segments. Irrespective ionic strength and micelle charge, the corona shrinks with increasing packing fraction. At high charge and minimal screening conditions, the polyelectrolyte chains remain almost fully stretched and they interdigitate once the volume fraction exceeds the critical value 0.53+/-0.02. Interpenetration of the polyelectrolyte brushes also controls the fluid rheology: The viscosity increases by three orders of magnitude and the parallel frequency scaling behavior of the dynamic moduli suggests the formation of a physical gel. In excess salt, the coronal layers are less extended and they do not interpenetrate in the present concentration range.
    Sprache Englisch
    Erscheinungsdatum 2005-01-08
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 3113-6
    ISSN 1089-7690 ; 0021-9606
    ISSN (online) 1089-7690
    ISSN 0021-9606
    DOI 10.1063/1.1829039
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  4. Artikel: Do spherical polyelectrolyte brushes interdigitate?

    Korobko, A V / Jesse, W / Egelhaaf, S U / Lapp, A / van der Maarel, J R C

    Physical review letters

    2004  Band 93, Heft 17, Seite(n) 177801

    Abstract: The structure of spherical micelles of the diblock copolymer poly(styrene-block-acrylic acid) in water was investigated with small angle neutron scattering and contrast matching. We have monitored intermicelle correlation and the extension of the ... ...

    Abstract The structure of spherical micelles of the diblock copolymer poly(styrene-block-acrylic acid) in water was investigated with small angle neutron scattering and contrast matching. We have monitored intermicelle correlation and the extension of the polyelectrolyte chains in the coronal layer through the overlap concentration. Irrespective of ionic strength, the corona shrinks with increasing packing fraction. Furthermore, at high charge and minimal screening conditions, the corona layers interpenetrate once the volume fraction exceeds the critical value 0.53+/-0.02.
    Sprache Englisch
    Erscheinungsdatum 2004-10-22
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 208853-8
    ISSN 1079-7114 ; 0031-9007
    ISSN (online) 1079-7114
    ISSN 0031-9007
    DOI 10.1103/PhysRevLett.93.177801
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  5. Artikel ; Online: Amplified stretch of bottlebrush-coated DNA in nanofluidic channels

    Zhang, C. / Hernandez Garcia, A. / Jiang, K. / Gong, Z.Y. / Guttula, D. / Ng, S.Y. / Malar, P.P. / van Kan, J.A. / Dai, L. / Doyle, P.S. / de Vries, R.J. / van der Maarel, J.R.C.

    Nucleic acids research

    2013  Band 41, Heft 20

    Abstract: The effect of a cationic-neutral diblock polypeptide on the conformation of single DNA molecules confined in rectangular nanochannels is investigated with fluorescence microscopy. An enhanced stretch along the channel is observed with increased binding ... ...

    Abstract The effect of a cationic-neutral diblock polypeptide on the conformation of single DNA molecules confined in rectangular nanochannels is investigated with fluorescence microscopy. An enhanced stretch along the channel is observed with increased binding of the cationic block of the polypeptide to DNA. A maximum stretch of 85% of the contour length can be achieved inside a channel with a cross-sectional diameter of 200 nm and at a 2-fold excess of polypeptide with respect to DNA charge. With site-specific fluorescence labelling, it is demonstrated that this maximum stretch is sufficient to map large-scale genomic organization. Monte Carlo computer simulation shows that the amplification of the stretch inside the nanochannels is owing to an increase in bending rigidity and thickness of bottlebrush-coated DNA. The persistence lengths and widths deduced from the nanochannel data agree with what has been estimated from the analysis of atomic force microscopy images of dried complexes on silica.
    Schlagwörter compaction ; confinement ; device ; force microscopy ; length ; nanochannel ; polymers ; protein ; single-molecule
    Thema/Rubrik (Code) 612
    Sprache Englisch
    Erscheinungsland nl
    Dokumenttyp Artikel ; Online
    ZDB-ID 186809-3
    ISSN 0301-5610 ; 0305-1048
    ISSN 0301-5610 ; 0305-1048
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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