Artikel ; Online: Intrarenal localization of the plasma membrane ATP channel pannexin1.
American journal of physiology. Renal physiology
2012 Band 303, Heft 10, Seite(n) F1454–9
Abstract: In the renal tubules, ATP released from epithelial cells stimulates purinergic receptors, regulating salt and water reabsorption. However, the mechanisms by which ATP is released into the tubular lumen are multifaceted. Pannexin1 (Panx1) is a newly ... ...
Abstract | In the renal tubules, ATP released from epithelial cells stimulates purinergic receptors, regulating salt and water reabsorption. However, the mechanisms by which ATP is released into the tubular lumen are multifaceted. Pannexin1 (Panx1) is a newly identified. ubiquitously expressed protein that forms connexin-like channels in the plasma membrane, which have been demonstrated to function as a mechanosensitive ATP conduit. Here, we report on the localization of Panx1 in the mouse kidney. Using immunofluorescence, strong Panx1 expression was observed in renal tubules, including proximal tubules, thin descending limbs, and collecting ducts, along their apical cell membranes. In the renal vasculature, Panx1 expression was localized to vascular smooth muscle cells in renal arteries, including the afferent and efferent arterioles. Additionally, we tested whether Panx1 channels expressed in renal epithelial cells facilitate luminal ATP release by measuring the ATP content of urine samples freshly collected from wild-type and Panx1(-/-) mice. Urinary ATP levels were reduced by 30% in Panx1(-/-) compared with wild-type mice. These results suggest that Panx1 channels in the kidney may regulate ATP release and via purinergic signaling may participate in the control of renal epithelial fluid and electrolyte transport and vascular functions. |
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Mesh-Begriff(e) | Adenosine Triphosphate/urine ; Animals ; Cell Membrane/metabolism ; Connexins/genetics ; Connexins/metabolism ; Epithelial Cells/metabolism ; Ion Channels/metabolism ; Kidney/metabolism ; Male ; Mice ; Mice, Knockout ; Nerve Tissue Proteins/genetics ; Nerve Tissue Proteins/metabolism ; Signal Transduction/physiology |
Chemische Substanzen | Connexins ; Ion Channels ; Nerve Tissue Proteins ; Panx1 protein, mouse ; Adenosine Triphosphate (8L70Q75FXE) |
Sprache | Englisch |
Erscheinungsdatum | 2012-09-05 |
Erscheinungsland | United States |
Dokumenttyp | Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't |
ZDB-ID | 603837-2 |
ISSN | 1522-1466 ; 0363-6127 |
ISSN (online) | 1522-1466 |
ISSN | 0363-6127 |
DOI | 10.1152/ajprenal.00206.2011 |
Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
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