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  1. Artikel ; Online: Seeing spermine blocking of K+ ion movement through inward rectifier Kir2.2 channels.

    Lee, Sun-Joo / Nichols, Colin G

    The Journal of general physiology

    2022  Band 155, Heft 2

    Abstract: Inwardly rectifier potassium (Kir) channels are a major potassium channel sub-class whose function is regulated by ligand-dependent gating and highly voltage-dependent block by polyamines. With molecular dynamics simulations over previously unattainable ... ...

    Abstract Inwardly rectifier potassium (Kir) channels are a major potassium channel sub-class whose function is regulated by ligand-dependent gating and highly voltage-dependent block by polyamines. With molecular dynamics simulations over previously unattainable timescales, Jogini et al. (J. Gen. Physiol. https://doi.org/10.1085/jgp.202213085) provide unprecedented visualization of K+ conduction through open Kir2.2 channels and of the molecular details of channel block by spermine.
    Mesh-Begriff(e) Spermine/pharmacology ; Ion Channel Gating ; Polyamines/pharmacology ; Potassium Channel Blockers/pharmacology ; Potassium/pharmacology
    Chemische Substanzen Spermine (2FZ7Y3VOQX) ; Polyamines ; Potassium Channel Blockers ; Potassium (RWP5GA015D)
    Sprache Englisch
    Erscheinungsdatum 2022-12-16
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural ; Comment
    ZDB-ID 3118-5
    ISSN 1540-7748 ; 0022-1295
    ISSN (online) 1540-7748
    ISSN 0022-1295
    DOI 10.1085/jgp.202213144
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel ; Online: Oxidation Driven Reversal of PIP

    Lee, Sun-Joo / Maeda, Shoji / Gao, Jian / Nichols, Colin G

    Function (Oxford, England)

    2023  Band 4, Heft 3, Seite(n) zqad016

    Abstract: Physiological activity of G protein gated inward rectifier ... ...

    Abstract Physiological activity of G protein gated inward rectifier K
    Mesh-Begriff(e) G Protein-Coupled Inwardly-Rectifying Potassium Channels/genetics ; GTP-Binding Proteins/metabolism ; Ligands ; Oxidation-Reduction
    Chemische Substanzen G Protein-Coupled Inwardly-Rectifying Potassium Channels ; GTP-Binding Proteins (EC 3.6.1.-) ; Ligands ; KCNJ6 protein, human
    Sprache Englisch
    Erscheinungsdatum 2023-04-10
    Erscheinungsland England
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural
    ISSN 2633-8823
    ISSN (online) 2633-8823
    DOI 10.1093/function/zqad016
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  3. Artikel ; Online: Development of Fluorescent Bacteria with Lux and Riboflavin Genes

    Sun-Joo Lim / Miae Choi / Inseop Yun / Seulgi Lee / Ny Chang / Chan-Yong Lee

    International Journal of Molecular Sciences, Vol 24, Iss 5096, p

    2023  Band 5096

    Abstract: Lumazine protein from marine luminescent bacteria of Photobacterium species bind with very high affinity to the fluorescent chromophore 6,7-dimethyl-8-ribitylumazine. The light emission of bacterial luminescent systems is used as a sensitive, rapid, and ... ...

    Abstract Lumazine protein from marine luminescent bacteria of Photobacterium species bind with very high affinity to the fluorescent chromophore 6,7-dimethyl-8-ribitylumazine. The light emission of bacterial luminescent systems is used as a sensitive, rapid, and safe assay for an ever-increasing number of biological systems. Plasmid pRFN4, containing the genes encoding riboflavin from the rib operon of Bacillus subtilis , was designed for the overproduction of lumazine. To construct fluorescent bacteria for use as microbial sensors, novel recombinant plasmids (pRFN4-Pp N- lum P and pRFN4-Pp lux LP N- lum P) were constructed by amplifying the DNA encoding the N- lum P gene ( lux L) from P. phosphoreum and the promoter region ( lux LP) present upstream of the lux operon of the gene by PCR and ligating into the pRFN4-Pp N- lum P plasmid. A new recombinant plasmid, pRFN4-Pp lux LP-N- lum P, was constructed with the expectation that the fluorescence intensity would be further increased when transformed into Escherichia coli . When this plasmid was transformed into E. coli 43R, the fluorescence intensity of transformants was 500 times greater than that of E. coli alone. As a result, the recombinant plasmid in which the gene encoding N-LumP and DNA containing the lux promoter exhibited expression that was so high as to show fluorescence in single E. coli cells. The fluorescent bacterial systems developed in the present study using lux and riboflavin genes can be utilized in the future as biosensors with high sensitivity and rapid analysis times.
    Schlagwörter bioluminescence ; fluorescence ; lux ; Photobacterium ; riboflavin ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Thema/Rubrik (Code) 572
    Sprache Englisch
    Erscheinungsdatum 2023-03-01T00:00:00Z
    Verlag MDPI AG
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  4. Artikel ; Online: Development of Fluorescent Bacteria with

    Lim, Sun-Joo / Choi, Miae / Yun, Inseop / Lee, Seulgi / Chang, Ny / Lee, Chan-Yong

    International journal of molecular sciences

    2023  Band 24, Heft 6

    Abstract: Lumazine protein from marine luminescent bacteria ... ...

    Abstract Lumazine protein from marine luminescent bacteria of
    Mesh-Begriff(e) Escherichia coli/genetics ; Escherichia coli/metabolism ; Riboflavin/metabolism ; Plasmids/genetics ; Promoter Regions, Genetic ; Operon ; Luminescent Measurements ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism
    Chemische Substanzen Riboflavin (TLM2976OFR) ; Bacterial Proteins
    Sprache Englisch
    Erscheinungsdatum 2023-03-07
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms24065096
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  5. Artikel ; Online: Perceptions of Patient Safety Culture and Medication Error Reporting among Early- and Mid-Career Female Nurses in South Korea

    Sun-Joo Jang / Haeyoung Lee / Youn-Jung Son

    International Journal of Environmental Research and Public Health, Vol 18, Iss 4853, p

    2021  Band 4853

    Abstract: Reporting medication errors is crucial for improving quality of care and patient safety in acute care settings. To date, little is known about how reporting varies between early and mid-career nurses. Thus, this study used a cross-sectional, secondary ... ...

    Abstract Reporting medication errors is crucial for improving quality of care and patient safety in acute care settings. To date, little is known about how reporting varies between early and mid-career nurses. Thus, this study used a cross-sectional, secondary data analysis design to investigate the differences between early (under the age of 35) and mid-career (ages 35–54) female nurses by examining their perceptions of patient safety culture using the Korean Hospital Survey on Patient Safety Culture (HSPSC) and single-item self-report measure of medication error reporting. A total of 311 hospital nurses (260 early-career and 51 mid-career nurses) completed questionnaires on perceived patient safety culture and medication error reporting. Early-career nurses had lower levels of perception regarding patient safety culture ( p = 0.034) compared to mid-career nurses. A multiple logistic regression analysis showed that relatively short clinical experience (<3 years) and a higher level of perceived patient safety culture increased the rate of appropriate medication error reporting among early-career nurses. However, there was no significant association between perception of patient safety culture and medication error reporting among mid-career nurses. Future studies should investigate the role of positive perception of patient safety culture on reporting errors considering multidimensional aspects, and include hospital contextual factors among early-, mid-, and late-career nurses.
    Schlagwörter nurses ; patient safety culture ; medication errors ; expertise ; nursing ; Medicine ; R
    Thema/Rubrik (Code) 300
    Sprache Englisch
    Erscheinungsdatum 2021-05-01T00:00:00Z
    Verlag MDPI AG
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  6. Artikel ; Online: Perceptions of Patient Safety Culture and Medication Error Reporting among Early- and Mid-Career Female Nurses in South Korea.

    Jang, Sun-Joo / Lee, Haeyoung / Son, Youn-Jung

    International journal of environmental research and public health

    2021  Band 18, Heft 9

    Abstract: Reporting medication errors is crucial for improving quality of care and patient safety in acute care settings. To date, little is known about how reporting varies between early and mid-career nurses. Thus, this study used a cross-sectional, secondary ... ...

    Abstract Reporting medication errors is crucial for improving quality of care and patient safety in acute care settings. To date, little is known about how reporting varies between early and mid-career nurses. Thus, this study used a cross-sectional, secondary data analysis design to investigate the differences between early (under the age of 35) and mid-career (ages 35-54) female nurses by examining their perceptions of patient safety culture using the Korean Hospital Survey on Patient Safety Culture (HSPSC) and single-item self-report measure of medication error reporting. A total of 311 hospital nurses (260 early-career and 51 mid-career nurses) completed questionnaires on perceived patient safety culture and medication error reporting. Early-career nurses had lower levels of perception regarding patient safety culture (
    Mesh-Begriff(e) Adult ; Cross-Sectional Studies ; Female ; Humans ; Medication Errors ; Middle Aged ; Nurses ; Nursing Staff, Hospital ; Organizational Culture ; Patient Safety ; Perception ; Republic of Korea ; Safety Management ; Surveys and Questionnaires
    Sprache Englisch
    Erscheinungsdatum 2021-05-01
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2175195-X
    ISSN 1660-4601 ; 1661-7827
    ISSN (online) 1660-4601
    ISSN 1661-7827
    DOI 10.3390/ijerph18094853
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  7. Artikel ; Online: Polyamines and potassium channels: A 25-year romance.

    Nichols, Colin G / Lee, Sun-Joo

    The Journal of biological chemistry

    2018  Band 293, Heft 48, Seite(n) 18779–18788

    Abstract: Potassium channels that exhibit the property of inward rectification (Kir channels) are present in most cells. Cloning of the first Kir channel genes 25 years ago led to recognition that inward rectification is a consequence of voltage-dependent block by ...

    Abstract Potassium channels that exhibit the property of inward rectification (Kir channels) are present in most cells. Cloning of the first Kir channel genes 25 years ago led to recognition that inward rectification is a consequence of voltage-dependent block by cytoplasmic polyamines, which are also ubiquitously present in animal cells. Upon cellular depolarization, these polycationic metabolites enter the Kir channel pore from the intracellular side, blocking the movement of K
    Mesh-Begriff(e) Ion Transport ; Polyamines/metabolism ; Potassium/metabolism ; Potassium Channels/chemistry ; Potassium Channels/genetics ; Potassium Channels/metabolism ; Protein Conformation
    Chemische Substanzen Polyamines ; Potassium Channels ; Potassium (RWP5GA015D)
    Sprache Englisch
    Erscheinungsdatum 2018-10-17
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural ; Review
    ZDB-ID 2997-x
    ISSN 1083-351X ; 0021-9258
    ISSN (online) 1083-351X
    ISSN 0021-9258
    DOI 10.1074/jbc.TM118.003344
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  8. Artikel ; Online: Subunit gating resulting from individual protonation events in Kir2 channels.

    Maksaev, Grigory / Bründl-Jirout, Michael / Stary-Weinzinger, Anna / Zangerl-Plessl, Eva-Maria / Lee, Sun-Joo / Nichols, Colin G

    Nature communications

    2023  Band 14, Heft 1, Seite(n) 4538

    Abstract: Inwardly rectifying potassium (Kir) channels open at the 'helix bundle crossing' (HBC), formed by the M2 helices at the cytoplasmic end of the transmembrane pore. Introduced negative charges at the HBC (G178D) in Kir2.2 channels forces opening, allowing ... ...

    Abstract Inwardly rectifying potassium (Kir) channels open at the 'helix bundle crossing' (HBC), formed by the M2 helices at the cytoplasmic end of the transmembrane pore. Introduced negative charges at the HBC (G178D) in Kir2.2 channels forces opening, allowing pore wetting and free movement of permeant ions between the cytoplasm and the inner cavity. Single-channel recordings reveal striking, pH-dependent, subconductance behaviors in G178D (or G178E and equivalent Kir2.1[G177E]) mutant channels, with well-resolved non-cooperative subconductance levels. Decreasing cytoplasmic pH shifts the probability towards lower conductance levels. Molecular dynamics simulations show how protonation of Kir2.2[G178D], or the D173 pore-lining residues, changes solvation, K
    Mesh-Begriff(e) Ions ; Molecular Dynamics Simulation ; Cytoplasm ; Oocytes
    Chemische Substanzen Ions
    Sprache Englisch
    Erscheinungsdatum 2023-07-28
    Erscheinungsland England
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-023-40058-7
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  9. Artikel: Subunit gating resulting from individual protonation events in Kir2 channels.

    Maksaev, Grigory / Bründl-Jirout, Michael / Stary-Weinzinger, Anna / Zangerl-Plessl, Eva-Maria / Lee, Sun-Joo / Nichols, Colin G

    Research square

    2023  

    Abstract: Inwardly rectifying potassium (Kir) channels play a critical role in stabilizing the membrane potential, thus controlling numerous physiological phenomena in multiple tissues. Channel conductance is activated by cytoplasmic modulators that open the ... ...

    Abstract Inwardly rectifying potassium (Kir) channels play a critical role in stabilizing the membrane potential, thus controlling numerous physiological phenomena in multiple tissues. Channel conductance is activated by cytoplasmic modulators that open the channel at the 'helix bundle crossing' (HBC), formed by the coming together of the M2 helices from each of the four subunits, at the cytoplasmic end of the transmembrane pore. We introduced a negative charge at the bundle crossing region (G178D) in classical inward rectifier Kir2.2 channel subunits that forces channel opening, allowing pore wetting and free movement of permeant ions between the cytoplasm and the inner cavity. Single-channel recordings reveal a striking pH-dependent subconductance behavior in G178D (or G178E and equivalent Kir2.1[G177E]) mutant channels that reflects individual subunit events. These subconductance levels are well resolved temporally and occur independently, with no evidence of cooperativity. Decreasing cytoplasmic pH shifts the probability towards lower conductance levels, and molecular dynamics simulations show how protonation of Kir2.2[G178D] and, additionally, the rectification controller (D173) pore-lining residues leads to changes in pore solvation, K+ ion occupancy, and ultimately K+ conductance. While subconductance gating has long been discussed, resolution and explanation have been lacking. The present data reveals how individual protonation events change the electrostatic microenvironment of the pore, resulting in distinct, uncoordinated, and relatively long-lasting conductance states, which depend on levels of ion pooling in the pore and the maintenance of pore wetting. Gating and conductance are classically understood as separate processes in ion channels. The remarkable sub-state gating behavior of these channels reveals how intimately connected 'gating' and 'conductance' are in reality.
    Sprache Englisch
    Erscheinungsdatum 2023-03-16
    Erscheinungsland United States
    Dokumenttyp Preprint
    DOI 10.21203/rs.3.rs-2640647/v1
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  10. Artikel: Prediction of Wildfire Fuel Load for Pinus densiflora Stands in South Korea Based on the Forest-Growth Model

    Lee, Sun-Joo / Lee, Young-Jin / Ryu, Ju-Yeol / Kwon, Chun-Geun / Seo, Kyung-Won / Kim, Sung-Yong

    Forests. 2022 Aug. 28, v. 13, no. 9

    2022  

    Abstract: A prediction model was developed for the wildfire fuel load of Korean red pine (Pinus densiflora) stands with susceptibility to forest fire based on the forest-growth model. Furthermore, a time-series analysis was performed on the variation in forest- ... ...

    Abstract A prediction model was developed for the wildfire fuel load of Korean red pine (Pinus densiflora) stands with susceptibility to forest fire based on the forest-growth model. Furthermore, a time-series analysis was performed on the variation in forest-fire fuel load according to forest management. National Forest Inventory stand data of 1434 plots for P. densiflora stands were used, and the final forest-fire fuel load prediction model was developed using the Weibull function and mortality model. The fit index of the diameter distribution model ranged from 0.58 (0th percentile) to 0.96 (50th percentile), and that of the mortality model was 0.68. The prediction of the stand growth variation after 20 years based on the growth data of managed and unmanaged stands indicated a mean stand density of 1518 trees per ha for unmanaged stands, and 885 trees per ha for managed stands. Regarding the variation in the available canopy fuel load distribution, the predicted annual increase was approximately 0.7 ton/ha for unmanaged stands and approximately 0.5 ton/ha for managed stands. These findings will contribute to setting fuel management criteria to prevent forest fire spread while providing the quantitative data of the characteristics of stand growth variation and the predicted wildfire fuel load.
    Schlagwörter Pinus densiflora ; Weibull statistics ; fire spread ; forest fires ; forest growth ; forest inventory ; forest management ; fuel loading ; fuels (fire ecology) ; models ; mortality ; national forests ; prediction ; stand density ; time series analysis ; South Korea
    Sprache Englisch
    Erscheinungsverlauf 2022-0828
    Erscheinungsort Multidisciplinary Digital Publishing Institute
    Dokumenttyp Artikel
    ZDB-ID 2527081-3
    ISSN 1999-4907
    ISSN 1999-4907
    DOI 10.3390/f13091372
    Datenquelle NAL Katalog (AGRICOLA)

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