Artikel ; Online: Development of an insulin-like growth factor-1 certified reference material by SI-traceable isotope-dilution mass spectrometry.
2024 Band 273, Seite(n) 125812
Abstract: In this study, an insulin-like growth factor-1 (IGF-1) certified reference material (CRM) was developed by the National Institute of Metrology (NIM), and two different principles for evaluating the IGF-1 CRM were established. After optimisation of the ... ...
Abstract | In this study, an insulin-like growth factor-1 (IGF-1) certified reference material (CRM) was developed by the National Institute of Metrology (NIM), and two different principles for evaluating the IGF-1 CRM were established. After optimisation of the acid hydrolysis conditions (110 °C, 36 h), quantitative determination of peptide purity, and chromatographic separation and mass spectrometric detection, amino acid analysis-based high-performance liquid chromatography combined with isotope-dilution tandem mass spectrometry (AAA-HPLC-IDMS/MS) and peptide analysis-based HPLC-IDMS/MS (Peptide-HPLC-IDMS/MS) were used for certified value assignment; the results obtained were 136.28 and 135.01 μg/g, respectively, which were in good agreement. These results were subjected to the normal distribution test, outlier test, and method consistency test. The homogeneity and stability of the reference materials were also examined, and the uncertainty introduced in the experimental process was calculated. The final certified value was (136 ± 15) μg g |
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Mesh-Begriff(e) | Insulin-Like Peptides ; Insulin-Like Growth Factor I ; Tandem Mass Spectrometry/methods ; Peptides ; Isotopes ; Reference Standards |
Chemische Substanzen | Insulin-Like Peptides ; Insulin-Like Growth Factor I (67763-96-6) ; Peptides ; Isotopes |
Sprache | Englisch |
Erscheinungsdatum | 2024-02-22 |
Erscheinungsland | Netherlands |
Dokumenttyp | Journal Article |
ZDB-ID | 1500969-5 |
ISSN | 1873-3573 ; 0039-9140 |
ISSN (online) | 1873-3573 |
ISSN | 0039-9140 |
DOI | 10.1016/j.talanta.2024.125812 |
Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
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