Artikel ; Online: Caspase-resistant ROCK1 expression prolongs survival of Eµ-Myc B cell lymphoma mice.
2024 Band 17, Heft 5
Abstract: Apoptosis is characterized by membrane blebbing and apoptotic body formation. Caspase cleavage of ROCK1 generates an active fragment that promotes actin-myosin-mediated contraction and membrane blebbing during apoptosis. Expression of caspase-resistant ... ...
Abstract | Apoptosis is characterized by membrane blebbing and apoptotic body formation. Caspase cleavage of ROCK1 generates an active fragment that promotes actin-myosin-mediated contraction and membrane blebbing during apoptosis. Expression of caspase-resistant non-cleavable ROCK1 (Rock1 NC) prolonged survival of mice that rapidly develop B cell lymphomas due to Eµ-Myc transgene expression. Eµ-Myc; Rock1 NC mice had significantly fewer bone marrow cells relative to those in Eµ-Myc mice expressing wild-type ROCK1 (Rock1 WT), which was associated with altered cell cycle profiles. Circulating macrophage numbers were lower in Eµ-Myc; Rock1 NC mice, but there were higher levels of bone marrow macrophages, consistent with spontaneous cell death in Eµ-Myc; Rock1 NC mouse bone marrows being more inflammatory. Rock1 WT recipient mice transplanted with pre-neoplastic Eµ-Myc; Rock1 NC bone marrow cells survived longer than mice transplanted with Eµ-Myc; Rock1 WT cells, indicating that the survival benefit was intrinsic to the Eµ-Myc; Rock1 NC bone marrow cells. The results suggest that the apoptotic death of Eµ-Myc; Rock1 NC cells generates a proliferation-suppressive microenvironment in bone marrows that reduces cell numbers and prolongs B cell lymphoma mouse survival. |
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Mesh-Begriff(e) | Animals ; rho-Associated Kinases/metabolism ; Lymphoma, B-Cell/pathology ; Lymphoma, B-Cell/genetics ; Proto-Oncogene Proteins c-myc/metabolism ; Caspases/metabolism ; Macrophages/metabolism ; Apoptosis ; Mice ; Survival Analysis ; Mice, Transgenic ; Bone Marrow Cells/metabolism ; Mice, Inbred C57BL ; Cell Cycle |
Chemische Substanzen | Rock1 protein, mouse |
Sprache | Englisch |
Erscheinungsdatum | 2024-05-21 |
Erscheinungsland | England |
Dokumenttyp | Journal Article |
ZDB-ID | 2451104-3 |
ISSN | 1754-8411 ; 1754-8403 |
ISSN (online) | 1754-8411 |
ISSN | 1754-8403 |
DOI | 10.1242/dmm.050631 |
Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
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