Artikel ; Online: Inflammation and bacteriophages affect DNA inversion states and functionality of the gut microbiota.
2024 Band 32, Heft 3, Seite(n) 322–334.e9
Abstract: Reversible genomic DNA inversions control the expression of numerous gut bacterial molecules, but how this impacts disease remains uncertain. By analyzing metagenomic samples from inflammatory bowel disease (IBD) cohorts, we identified multiple ... ...
Abstract | Reversible genomic DNA inversions control the expression of numerous gut bacterial molecules, but how this impacts disease remains uncertain. By analyzing metagenomic samples from inflammatory bowel disease (IBD) cohorts, we identified multiple invertible regions where a particular orientation correlated with disease. These include the promoter of polysaccharide A (PSA) of Bacteroides fragilis, which induces regulatory T cells (Tregs) and ameliorates experimental colitis. The PSA promoter was mostly oriented "OFF" in IBD patients, which correlated with increased B. fragilis-associated bacteriophages. Similarly, in mice colonized with a healthy human microbiota and B. fragilis, induction of colitis caused a decline of PSA in the "ON" orientation that reversed as inflammation resolved. Monocolonization of mice with B. fragilis revealed that bacteriophage infection increased the frequency of PSA in the "OFF" orientation, causing reduced PSA expression and decreased Treg cells. Altogether, we reveal dynamic bacterial phase variations driven by bacteriophages and host inflammation, signifying bacterial functional plasticity during disease. |
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Mesh-Begriff(e) | Humans ; Animals ; Mice ; Gastrointestinal Microbiome ; Colitis ; Inflammatory Bowel Diseases/microbiology ; Inflammation ; DNA |
Chemische Substanzen | DNA (9007-49-2) |
Sprache | Englisch |
Erscheinungsdatum | 2024-02-28 |
Erscheinungsland | United States |
Dokumenttyp | Journal Article |
ZDB-ID | 2278004-X |
ISSN | 1934-6069 ; 1931-3128 |
ISSN (online) | 1934-6069 |
ISSN | 1931-3128 |
DOI | 10.1016/j.chom.2024.02.003 |
Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
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