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  1. Artikel ; Online: Impact of improving infection control and antibiotic stewardship practices on nosocomial infections and antimicrobial resistance in an oncology centre from India.

    Bansal, Nitin / Goyal, Pankaj / Basu, Dharmishtha / Batra, Ullas / Sachdeva, Neelam / Joga, Srujana / Jain, Arpit / Doval, D C

    Indian journal of medical microbiology

    2023  Band 45, Seite(n) 100383

    Abstract: Background: Improving basic infection control (IC) practices, diagnostics and anti-microbial stewardship (AMS) are key tools to handle antimicrobial resistance (AMR).: Materials and methods: This is a retrospective study done over 6 years (2016-2021) ...

    Abstract Background: Improving basic infection control (IC) practices, diagnostics and anti-microbial stewardship (AMS) are key tools to handle antimicrobial resistance (AMR).
    Materials and methods: This is a retrospective study done over 6 years (2016-2021) in an oncology centre in North India with many on-going interventions to improve IC practices, diagnostics and AMS. This study looked into AMR patterns from clinical isolates, rates of hospital acquired infections (HAI) and clinical outcomes.
    Results: Over all, 98,915 samples were sent for culture from 158,191 admitted patients. Most commonly isolated organism was E. coli (n ​= ​6951; 30.1%) followed by Klebsiella pneumoniae (n ​= ​5801; 25.1%) and Pseudomonas aeroginosa (n ​= ​3041; 13.1%). VRE (Vancomycin resistant Enterococcus) rates fell down from 43.5% in Jan-June 2016 to 12.2% in July-Dec 2021, same was seen in CR (carbapenem resistant) Pseudomonas (23.0%-20.6%, CR Acinetobacter (66.6%-17.02%) and CR E. coli (21.6%-19.4%) over the same study period. Rate of isolation of Candida spp. from non-sterile sites also showed reduction (1.68 per 100 patients to 0.65 per 100 patients). Incidence of health care associated infections also fell from 2.3 to 1.19 per 1000 line days for CLABSI, 2.28 to 1.88 per 1000 catheter days for CAUTI. There was no change in overall mortality rates across the study period.
    Conclusion: This study emphasizes the point that improving compliance to standard IC recommendations and improving diagnostics can help in reducing the burden of antimicrobial resistance.
    Mesh-Begriff(e) Humans ; Cross Infection/drug therapy ; Cross Infection/epidemiology ; Cross Infection/etiology ; Anti-Bacterial Agents/therapeutic use ; Anti-Bacterial Agents/pharmacology ; Escherichia coli ; Antimicrobial Stewardship ; Retrospective Studies ; Drug Resistance, Bacterial ; Infection Control
    Chemische Substanzen Anti-Bacterial Agents
    Sprache Englisch
    Erscheinungsdatum 2023-05-23
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 1038798-5
    ISSN 1998-3646 ; 0255-0857
    ISSN (online) 1998-3646
    ISSN 0255-0857
    DOI 10.1016/j.ijmmb.2023.100383
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  2. Artikel ; Online: Module to Support Real-Time Microscopic Imaging of Living Organisms on Ground-Based Microgravity Analogs

    Srujana Neelam / Audrey Lee / Michael A. Lane / Ceasar Udave / Howard G. Levine / Ye Zhang

    Applied Sciences, Vol 11, Iss 3122, p

    2021  Band 3122

    Abstract: Since opportunities for spaceflight experiments are scarce, ground-based microgravity simulation devices (MSDs) offer accessible and economical alternatives for gravitational biology studies. Among the MSDs, the random positioning machine (RPM) provides ... ...

    Abstract Since opportunities for spaceflight experiments are scarce, ground-based microgravity simulation devices (MSDs) offer accessible and economical alternatives for gravitational biology studies. Among the MSDs, the random positioning machine (RPM) provides simulated microgravity conditions on the ground by randomizing rotating biological samples in two axes to distribute the Earth’s gravity vector in all directions over time. Real-time microscopy and image acquisition during microgravity simulation are of particular interest to enable the study of how basic cell functions, such as division, migration, and proliferation, progress under altered gravity conditions. However, these capabilities have been difficult to implement due to the constantly moving frames of the RPM as well as mechanical noise. Therefore, we developed an image acquisition module that can be mounted on an RPM to capture live images over time while the specimen is in the simulated microgravity (SMG) environment. This module integrates a digital microscope with a magnification range of 20× to 700×, a high-speed data transmission adaptor for the wireless streaming of time-lapse images, and a backlight illuminator to view the sample under brightfield and darkfield modes. With this module, we successfully demonstrated the real-time imaging of human cells cultured on an RPM in brightfield, lasting up to 80 h, and also visualized them in green fluorescent channel. This module was successful in monitoring cell morphology and in quantifying the rate of cell division, cell migration, and wound healing in SMG. It can be easily modified to study the response of other biological specimens to SMG.
    Schlagwörter microgravity simulation ; cell culture ; live cell imaging ; random positioning machine ; Technology ; T ; Engineering (General). Civil engineering (General) ; TA1-2040 ; Biology (General) ; QH301-705.5 ; Physics ; QC1-999 ; Chemistry ; QD1-999
    Thema/Rubrik (Code) 600
    Sprache Englisch
    Erscheinungsdatum 2021-04-01T00:00:00Z
    Verlag MDPI AG
    Dokumenttyp Artikel ; Online
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  3. Artikel: Simulated galactic cosmic ray exposure activates dose-dependent DNA repair response and down regulates glucosinolate pathways in arabidopsis seedlings.

    Dixit, Anirudha R / Meyers, Alexander D / Richardson, Brian / Richards, Jeffrey T / Richards, Stephanie E / Neelam, Srujana / Levine, Howard G / Cameron, Mark J / Zhang, Ye

    Frontiers in plant science

    2023  Band 14, Seite(n) 1284529

    Abstract: Outside the protection of Earth's magnetic field, organisms are constantly exposed to space radiation consisting of energetic protons and other heavier charged particles. With the goal of crewed Mars exploration, the production of fresh food during long ... ...

    Abstract Outside the protection of Earth's magnetic field, organisms are constantly exposed to space radiation consisting of energetic protons and other heavier charged particles. With the goal of crewed Mars exploration, the production of fresh food during long duration space missions is critical for meeting astronauts' nutritional and psychological needs. However, the biological effects of space radiation on plants have not been sufficiently investigated and characterized. To that end, 10-day-old
    Sprache Englisch
    Erscheinungsdatum 2023-12-14
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2613694-6
    ISSN 1664-462X
    ISSN 1664-462X
    DOI 10.3389/fpls.2023.1284529
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  4. Artikel: Response of

    Zhang, Ye / Richards, Jeffrey T / Feiveson, Alan H / Richards, Stephanie E / Neelam, Srujana / Dreschel, Thomas W / Plante, Ianik / Hada, Megumi / Wu, Honglu / Massa, Gioia D / Douglas, Grace L / Levine, Howard G

    Life (Basel, Switzerland)

    2022  Band 12, Heft 2

    Abstract: One of the major concerns for long-term exploration missions beyond the Earth's magnetosphere is consequences from exposures to solar particle event (SPE) protons and galactic cosmic rays (GCR). For long-term crewed Lunar and Mars explorations, the ... ...

    Abstract One of the major concerns for long-term exploration missions beyond the Earth's magnetosphere is consequences from exposures to solar particle event (SPE) protons and galactic cosmic rays (GCR). For long-term crewed Lunar and Mars explorations, the production of fresh food in space will provide both nutritional supplements and psychological benefits to the astronauts. However, the effects of space radiation on plants and plant propagules have not been sufficiently investigated and characterized. In this study, we evaluated the effect of two different compositions of charged particles-simulated GCR, and simulated SPE protons on dry and hydrated seeds of the model plant
    Sprache Englisch
    Erscheinungsdatum 2022-01-19
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2662250-6
    ISSN 2075-1729
    ISSN 2075-1729
    DOI 10.3390/life12020144
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  5. Artikel ; Online: New approaches for understanding the nuclear force balance in living, adherent cells.

    Neelam, Srujana / Dickinson, Richard B / Lele, Tanmay P

    Methods (San Diego, Calif.)

    2016  Band 94, Seite(n) 27–32

    Abstract: Cytoskeletal forces are transmitted to the nucleus to position and shape it. Linkages mediated by the LINC (linker of nucleoskeleton and cytoskeleton) complex transfer these forces to the nuclear envelope. Nuclear position and shape can be thought to be ... ...

    Abstract Cytoskeletal forces are transmitted to the nucleus to position and shape it. Linkages mediated by the LINC (linker of nucleoskeleton and cytoskeleton) complex transfer these forces to the nuclear envelope. Nuclear position and shape can be thought to be determined by a balance of cytoskeletal forces generated by microtubule motors that shear the nuclear surface, actomyosin forces that can pull, push and shear the nucleus, and intermediate filaments that may passively resist nuclear decentering and deformation. Parsing contributions of these different forces to nuclear mechanics is a very challenging task. Here we review new approaches that can be used in living cells to probe and understand the nuclear force balance.
    Mesh-Begriff(e) Cell Adhesion ; Cell Movement ; Cell Nucleus/physiology ; Cytoskeleton/physiology ; Humans ; Microscopy, Atomic Force
    Sprache Englisch
    Erscheinungsdatum 2016-02-01
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S. ; Review
    ZDB-ID 1066584-5
    ISSN 1095-9130 ; 1046-2023
    ISSN (online) 1095-9130
    ISSN 1046-2023
    DOI 10.1016/j.ymeth.2015.06.014
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  6. Artikel: New approaches for understanding the nuclear force balance in living, adherent cells

    Neelam, Srujana / Richard B. Dickinson / Tanmay P. Lele

    Methods. 2016 Feb. 01, v. 94

    2016  

    Abstract: Cytoskeletal forces are transmitted to the nucleus to position and shape it. Linkages mediated by the LINC (linker of nucleoskeleton and cytoskeleton) complex transfer these forces to the nuclear envelope. Nuclear position and shape can be thought to be ... ...

    Abstract Cytoskeletal forces are transmitted to the nucleus to position and shape it. Linkages mediated by the LINC (linker of nucleoskeleton and cytoskeleton) complex transfer these forces to the nuclear envelope. Nuclear position and shape can be thought to be determined by a balance of cytoskeletal forces generated by microtubule motors that shear the nuclear surface, actomyosin forces that can pull, push and shear the nucleus, and intermediate filaments that may passively resist nuclear decentering and deformation. Parsing contributions of these different forces to nuclear mechanics is a very challenging task. Here we review new approaches that can be used in living cells to probe and understand the nuclear force balance.
    Schlagwörter deformation ; intermediate filaments ; microtubules ; nuclear membrane
    Sprache Englisch
    Erscheinungsverlauf 2016-0201
    Umfang p. 27-32.
    Erscheinungsort Elsevier Inc.
    Dokumenttyp Artikel
    ZDB-ID 1066584-5
    ISSN 1095-9130 ; 1046-2023
    ISSN (online) 1095-9130
    ISSN 1046-2023
    DOI 10.1016/j.ymeth.2015.06.014
    Datenquelle NAL Katalog (AGRICOLA)

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  7. Artikel ; Online: Response of Arabidopsis thaliana and Mizuna Mustard Seeds to Simulated Space Radiation Exposures

    Ye Zhang / Jeffrey T. Richards / Alan H. Feiveson / Stephanie E. Richards / Srujana Neelam / Thomas W. Dreschel / Ianik Plante / Megumi Hada / Honglu Wu / Gioia D. Massa / Grace L. Douglas / Howard G. Levine

    Life, Vol 12, Iss 144, p

    2022  Band 144

    Abstract: One of the major concerns for long-term exploration missions beyond the Earth’s magnetosphere is consequences from exposures to solar particle event (SPE) protons and galactic cosmic rays (GCR). For long-term crewed Lunar and Mars explorations, the ... ...

    Abstract One of the major concerns for long-term exploration missions beyond the Earth’s magnetosphere is consequences from exposures to solar particle event (SPE) protons and galactic cosmic rays (GCR). For long-term crewed Lunar and Mars explorations, the production of fresh food in space will provide both nutritional supplements and psychological benefits to the astronauts. However, the effects of space radiation on plants and plant propagules have not been sufficiently investigated and characterized. In this study, we evaluated the effect of two different compositions of charged particles-simulated GCR, and simulated SPE protons on dry and hydrated seeds of the model plant Arabidopsis thaliana and the crop plant Mizuna mustard [ Brassica rapa var. japonica ]. Exposures to charged particles, simulated GCRs (up to 80 cGy) or SPEs (up to 200 cGy), were performed either acutely or at a low dose rate using the NASA Space Radiation Laboratory (NSRL) facility at Brookhaven National Lab (BNL). Control and irradiated seeds were planted in a solid phytogel and grown in a controlled environment. Five to seven days after planting, morphological parameters were measured to evaluate radiation-induced damage in the seedlings. After exposure to single types of charged particles, as well as to simulated GCR, the hydrated Arabidopsis seeds showed dose- and quality-dependent responses, with heavier ions causing more severe defects. Seeds exposed to simulated GCR (dry seeds) and SPE (hydrated seeds) had significant, although much less damage than seeds exposed to heavier and higher linear energy transfer (LET) particles. In general, the extent of damage depends on the seed type.
    Schlagwörter space radiation ; galactic cosmic rays ; solar particle event ; Arabidopsis ; mizuna mustard ; seeds ; Science ; Q
    Thema/Rubrik (Code) 580
    Sprache Englisch
    Erscheinungsdatum 2022-01-01T00:00:00Z
    Verlag MDPI AG
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  8. Artikel: Esophageal Melanocytosis - An Unusual Melanocytic Entity.

    Kumari, Neelam S / Srujana, Shyamala / Sireesha, Annapoorna / Krishna, L / Kumar, O Sharavan

    The Journal of the Association of Physicians of India

    2016  Band 64, Heft 5, Seite(n) 75–76

    Abstract: Esophageal melanocytosis is a rare benign condition characterized by melanocytic proliferation in esophageal squamous epithelium and melanin deposition in the mucosa. Because of its uncommon nature, pathologists and gastroenterologists lack experience ... ...

    Abstract Esophageal melanocytosis is a rare benign condition characterized by melanocytic proliferation in esophageal squamous epithelium and melanin deposition in the mucosa. Because of its uncommon nature, pathologists and gastroenterologists lack experience with this entity. We present a case of esophageal melanocytosis in a 66 years old male patient who presented with atypical chest pain and dysphagia. Endoscopic guided biopsy was done, provisional diagnosis of esophageal melanocytosis was made. Bleaching and immunohistochemistry confirmed the diagnosis.
    Mesh-Begriff(e) Aged ; Biopsy ; Deglutition Disorders ; Endoscopy, Digestive System/methods ; Esophageal Diseases/pathology ; Esophagus ; Humans ; Male ; Melanins/metabolism ; Melanocytes/metabolism ; Melanocytes/pathology ; Melanosis/metabolism ; Melanosis/pathology
    Chemische Substanzen Melanins
    Sprache Englisch
    Erscheinungsdatum 2016-10-12
    Erscheinungsland India
    Dokumenttyp Case Reports
    ZDB-ID 800766-4
    ISSN 0004-5772
    ISSN 0004-5772
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  9. Artikel ; Online: Changes in Nuclear Shape and Gene Expression in Response to Simulated Microgravity Are LINC Complex-Dependent

    Srujana Neelam / Brian Richardson / Richard Barker / Ceasar Udave / Simon Gilroy / Mark Cameron / Howard G. Levine / Ye Zhang

    International Journal of Molecular Sciences, Vol 21, Iss 6762, p

    2020  Band 6762

    Abstract: Microgravity is known to affect the organization of the cytoskeleton, cell and nuclear morphology and to elicit differential expression of genes associated with the cytoskeleton, focal adhesions and the extracellular matrix. Although the nucleus is ... ...

    Abstract Microgravity is known to affect the organization of the cytoskeleton, cell and nuclear morphology and to elicit differential expression of genes associated with the cytoskeleton, focal adhesions and the extracellular matrix. Although the nucleus is mechanically connected to the cytoskeleton through the Linker of Nucleoskeleton and Cytoskeleton (LINC) complex, the role of this group of proteins in these responses to microgravity has yet to be defined. In our study, we used a simulated microgravity device, a 3-D clinostat (Gravite), to investigate whether the LINC complex mediates cellular responses to the simulated microgravity environment. We show that nuclear shape and differential gene expression are both responsive to simulated microgravity in a LINC-dependent manner and that this response changes with the duration of exposure to simulated microgravity. These LINC-dependent genes likely represent elements normally regulated by the mechanical forces imposed by gravity on Earth.
    Schlagwörter LINC complex ; simulated microgravity ; nuclear morphology ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Thema/Rubrik (Code) 612
    Sprache Englisch
    Erscheinungsdatum 2020-09-01T00:00:00Z
    Verlag MDPI AG
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  10. Artikel ; Online: Changes in Nuclear Shape and Gene Expression in Response to Simulated Microgravity Are LINC Complex-Dependent.

    Neelam, Srujana / Richardson, Brian / Barker, Richard / Udave, Ceasar / Gilroy, Simon / Cameron, Mark J / Levine, Howard G / Zhang, Ye

    International journal of molecular sciences

    2020  Band 21, Heft 18

    Abstract: Microgravity is known to affect the organization of the cytoskeleton, cell and nuclear morphology and to elicit differential expression of genes associated with the cytoskeleton, focal adhesions and the extracellular matrix. Although the nucleus is ... ...

    Abstract Microgravity is known to affect the organization of the cytoskeleton, cell and nuclear morphology and to elicit differential expression of genes associated with the cytoskeleton, focal adhesions and the extracellular matrix. Although the nucleus is mechanically connected to the cytoskeleton through the Linker of Nucleoskeleton and Cytoskeleton (LINC) complex, the role of this group of proteins in these responses to microgravity has yet to be defined. In our study, we used a simulated microgravity device, a 3-D clinostat (Gravite), to investigate whether the LINC complex mediates cellular responses to the simulated microgravity environment. We show that nuclear shape and differential gene expression are both responsive to simulated microgravity in a LINC-dependent manner and that this response changes with the duration of exposure to simulated microgravity. These LINC-dependent genes likely represent elements normally regulated by the mechanical forces imposed by gravity on Earth.
    Mesh-Begriff(e) Cell Line ; Cell Nucleus/physiology ; Cytoskeleton/physiology ; Extracellular Matrix/physiology ; Focal Adhesions/physiology ; Gene Expression/physiology ; Humans ; Nuclear Matrix/physiology ; Weightlessness ; Weightlessness Simulation/methods
    Sprache Englisch
    Erscheinungsdatum 2020-09-15
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms21186762
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