LIVIVO - Suchergebnisse -

Suchergebnis

Treffer 1 - 10 von insgesamt 45

Suchoptionen

Artikel ; Online: Next-generation microfluidic point-of-care diagnostics.

Ng, Alphonsus H C / Wheeler, Aaron R

2015 Band 61, Heft 10, Seite(n) 1233–1234

Mesh-Begriff(e)	Clinical Chemistry Tests/instrumentation ; Equipment Design ; Humans ; Lab-On-A-Chip Devices ; Microfluidic Analytical Techniques/instrumentation ; Point-of-Care Systems ; Smartphone/instrumentation
Sprache	Englisch
Erscheinungsdatum	2015-05-05
Erscheinungsland	England
Dokumenttyp	Journal Article
ZDB-ID	80102-1
ISSN	1530-8561 ; 0009-9147
ISSN (online)	1530-8561
ISSN	0009-9147
DOI	10.1373/clinchem.2015.240226
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

Zusatzmaterialien

Verfügbar in ZB MED Köln/Königswinter

Ud II Zs.171: Hefte anzeigen

Standort:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Über subito bestellen

Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

Details ▾
- Im ZB MED-Bestand
- Kostenpflichtig bestellen

Artikel ; Online: Large libraries of single-chain trimer peptide-MHCs enable antigen-specific CD8+ T cell discovery and analysis.

Chour, William / Choi, Jongchan / Xie, Jingyi / Chaffee, Mary E / Schmitt, Thomas M / Finton, Kathryn / DeLucia, Diana C / Xu, Alexander M / Su, Yapeng / Chen, Daniel G / Zhang, Rongyu / Yuan, Dan / Hong, Sunga / Ng, Alphonsus H C / Butler, Jonah Z / Edmark, Rick A / Jones, Lesley C / Murray, Kim M / Peng, Songming /

Li, Guideng / Strong, Roland K / Lee, John K / Goldman, Jason D / Greenberg, Philip D / Heath, James R

Communications biology

2023 Band 6, Heft 1, Seite(n) 528

Abstract: The discovery and characterization of antigen-specific ... ...

Abstract	The discovery and characterization of antigen-specific CD8
Mesh-Begriff(e)	Humans ; CD8-Positive T-Lymphocytes ; COVID-19 ; SARS-CoV-2/genetics ; Antigens ; Epitopes ; Peptides/genetics
Chemische Substanzen	Antigens ; Epitopes ; Peptides
Sprache	Englisch
Erscheinungsdatum	2023-05-16
Erscheinungsland	England
Dokumenttyp	Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, N.I.H., Extramural
ISSN	2399-3642
ISSN (online)	2399-3642
DOI	10.1038/s42003-023-04899-8
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

Zusatzmaterialien

Über subito bestellen

Fernleihe an ZB MED

Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

Details ▾
- Kostenpflichtig bestellen

Artikel ; Online: Resolution of tissue signatures of therapy response in patients with recurrent GBM treated with neoadjuvant anti-PD1

Yue Lu / Alphonsus H. C. Ng / Frances E. Chow / Richard G. Everson / Beth A. Helmink / Michael T. Tetzlaff / Rohit Thakur / Jennifer A. Wargo / Timothy F. Cloughesy / Robert M. Prins / James R. Heath

Nature Communications, Vol 12, Iss 1, Pp 1-

2021 Band 13

Abstract: The response to neoadjuvant immune checkpoint blockade (ICB) in patients with recurrent gliolastoma multiforme (GBM) has been challenging to interpret. Here the authors develop a tumor analysis framework that reveals molecular similarities between GBM ... ...

Abstract	The response to neoadjuvant immune checkpoint blockade (ICB) in patients with recurrent gliolastoma multiforme (GBM) has been challenging to interpret. Here the authors develop a tumor analysis framework that reveals molecular similarities between GBM and melanoma and unique patterns of immunosuppression in GBM indicating potential co-targets for neoadjuvant ICB.
Schlagwörter	Science ; Q
Sprache	Englisch
Erscheinungsdatum	2021-06-01T00:00:00Z
Verlag	Nature Portfolio
Dokumenttyp	Artikel ; Online
Datenquelle	BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

Volltext online

Volltext online

Zusatzmaterialien

Fernleihe an ZB MED

Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

Artikel ; Online: Organ-specific immunity: A tissue analysis framework for investigating local immune responses to SARS-CoV-2.

Cell reports

2023 Band 42, Heft 10, Seite(n) 113212

Abstract: Local immune activation at mucosal surfaces, mediated by mucosal lymphoid tissues, is vital for effective immune responses against pathogens. While pathogens like severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can spread to multiple organs, ...

Abstract	Local immune activation at mucosal surfaces, mediated by mucosal lymphoid tissues, is vital for effective immune responses against pathogens. While pathogens like severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can spread to multiple organs, patients with coronavirus disease 2019 (COVID-19) primarily experience inflammation and damage in their lungs. To investigate this apparent organ-specific immune response, we develop an analytical framework that recognizes the significance of mucosal lymphoid tissues. This framework combines histology, immunofluorescence, spatial transcript profiling, and mathematical modeling to identify cellular and gene expression differences between the lymphoid tissues of the lung and the gut and predict the determinants of those differences. Our findings indicate that mucosal lymphoid tissues are pivotal in organ-specific immune response to SARS-CoV-2, mediating local inflammation and tissue damage and contributing to immune dysfunction. The framework developed here has potential utility in the study of long COVID and may streamline biomarker discovery and treatment design for diseases with differential pathologies at the organ level.
Mesh-Begriff(e)	Humans ; SARS-CoV-2 ; COVID-19 ; Post-Acute COVID-19 Syndrome ; Inflammation ; Immunity
Sprache	Englisch
Erscheinungsdatum	2023-10-04
Erscheinungsland	United States
Dokumenttyp	Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, Non-U.S. Gov't
ZDB-ID	2649101-1
ISSN	2211-1247 ; 2211-1247
ISSN (online)	2211-1247
ISSN	2211-1247
DOI	10.1016/j.celrep.2023.113212
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

Zusatzmaterialien

Über subito bestellen

Details ▾
- Kostenpflichtig bestellen

Artikel ; Online: Raman-guided subcellular pharmaco-metabolomics for metastatic melanoma cells

Jiajun Du / Yapeng Su / Chenxi Qian / Dan Yuan / Kun Miao / Dongkwan Lee / Alphonsus H. C. Ng / Reto S. Wijker / Antoni Ribas / Raphael D. Levine / James R. Heath / Lu Wei

Nature Communications, Vol 11, Iss 1, Pp 1-

2020 Band 16

Abstract: Single-cell metabolomics can offer deep insights into the metabolic reprogramming that accompanies disease states. Here, the authors use Raman spectro-microscopy for non-invasive metabolite analysis and identification of druggable metabolic ... ...

Abstract	Single-cell metabolomics can offer deep insights into the metabolic reprogramming that accompanies disease states. Here, the authors use Raman spectro-microscopy for non-invasive metabolite analysis and identification of druggable metabolic susceptibilities in single live melanoma cells.
Schlagwörter	Science ; Q
Sprache	Englisch
Erscheinungsdatum	2020-09-01T00:00:00Z
Verlag	Nature Publishing Group
Dokumenttyp	Artikel ; Online
Datenquelle	BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

Volltext online

Volltext online

Zusatzmaterialien

Fernleihe an ZB MED

Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

Artikel ; Online: Raman-guided subcellular pharmaco-metabolomics for metastatic melanoma cells

Jiajun Du / Yapeng Su / Chenxi Qian / Dan Yuan / Kun Miao / Dongkwan Lee / Alphonsus H. C. Ng / Reto S. Wijker / Antoni Ribas / Raphael D. Levine / James R. Heath / Lu Wei

Nature Communications, Vol 11, Iss 1, Pp 1-

2020 Band 16

Abstract	Single-cell metabolomics can offer deep insights into the metabolic reprogramming that accompanies disease states. Here, the authors use Raman spectro-microscopy for non-invasive metabolite analysis and identification of druggable metabolic susceptibilities in single live melanoma cells.
Schlagwörter	Science ; Q
Sprache	Englisch
Erscheinungsdatum	2020-09-01T00:00:00Z
Verlag	Nature Portfolio
Dokumenttyp	Artikel ; Online
Datenquelle	BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

Volltext online

Volltext online

Zusatzmaterialien

Fernleihe an ZB MED

Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

Artikel ; Online: Resolution of tissue signatures of therapy response in patients with recurrent GBM treated with neoadjuvant anti-PD1.

Lu, Yue / Ng, Alphonsus H C / Chow, Frances E / Everson, Richard G / Helmink, Beth A / Tetzlaff, Michael T / Thakur, Rohit / Wargo, Jennifer A / Cloughesy, Timothy F / Prins, Robert M / Heath, James R

Nature communications

2021 Band 12, Heft 1, Seite(n) 4031

Abstract: The response of patients with recurrent glioblastoma multiforme to neoadjuvant immune checkpoint blockade has been challenging to interpret due to the inter-patient and intra-tumor heterogeneity. We report on a comparative analysis of tumor tissues ... ...

Abstract	The response of patients with recurrent glioblastoma multiforme to neoadjuvant immune checkpoint blockade has been challenging to interpret due to the inter-patient and intra-tumor heterogeneity. We report on a comparative analysis of tumor tissues collected from patients with recurrent glioblastoma and high-risk melanoma, both treated with neoadjuvant checkpoint blockade. We develop a framework that uses multiplex spatial protein profiling, machine learning-based image analysis, and data-driven computational models to investigate the pathophysiological and molecular factors within the tumor microenvironment that influence treatment response. Using melanoma to guide the interpretation of glioblastoma analyses, we interrogate the protein expression in microscopic compartments of tumors, and determine the correlates of cytotoxic CD8+ T cells, tumor growth, treatment response, and immune cell-cell interaction. This work reveals similarities shared between glioblastoma and melanoma, immunosuppressive factors that are unique to the glioblastoma microenvironment, and potential co-targets for enhancing the efficacy of neoadjuvant immune checkpoint blockade.
Mesh-Begriff(e)	Adult ; Aged ; Antineoplastic Agents, Immunological/therapeutic use ; Biomarkers, Tumor/analysis ; Brain Neoplasms/drug therapy ; Brain Neoplasms/pathology ; CD8-Positive T-Lymphocytes/immunology ; CTLA-4 Antigen/antagonists & inhibitors ; Female ; Glioblastoma/drug therapy ; Glioblastoma/pathology ; Humans ; Immune Checkpoint Inhibitors/therapeutic use ; Ipilimumab/therapeutic use ; Male ; Melanoma/drug therapy ; Middle Aged ; Neoadjuvant Therapy ; Neoplasm Recurrence, Local/drug therapy ; Neoplasm Recurrence, Local/pathology ; Nivolumab/therapeutic use ; Programmed Cell Death 1 Receptor/antagonists & inhibitors ; Treatment Outcome ; Tumor Microenvironment/immunology
Chemische Substanzen	Antineoplastic Agents, Immunological ; Biomarkers, Tumor ; CTLA-4 Antigen ; CTLA4 protein, human ; Immune Checkpoint Inhibitors ; Ipilimumab ; PDCD1 protein, human ; Programmed Cell Death 1 Receptor ; Nivolumab (31YO63LBSN)
Sprache	Englisch
Erscheinungsdatum	2021-06-29
Erscheinungsland	England
Dokumenttyp	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2553671-0
ISSN	2041-1723 ; 2041-1723
ISSN (online)	2041-1723
ISSN	2041-1723
DOI	10.1038/s41467-021-24293-4
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

Zusatzmaterialien

Über subito bestellen

Details ▾
- Kostenpflichtig bestellen

Artikel ; Online: Phenotypic heterogeneity and evolution of melanoma cells associated with targeted therapy resistance.

Su, Yapeng / Bintz, Marcus / Yang, Yezi / Robert, Lidia / Ng, Alphonsus H C / Liu, Victoria / Ribas, Antoni / Heath, James R / Wei, Wei

PLoS computational biology

2019 Band 15, Heft 6, Seite(n) e1007034

Abstract: Phenotypic plasticity is associated with non-genetic drug tolerance in several cancers. Such plasticity can arise from chromatin remodeling, transcriptomic reprogramming, and/or protein signaling rewiring, and is characterized as a cell state transition ... ...

Abstract	Phenotypic plasticity is associated with non-genetic drug tolerance in several cancers. Such plasticity can arise from chromatin remodeling, transcriptomic reprogramming, and/or protein signaling rewiring, and is characterized as a cell state transition in response to molecular or physical perturbations. This, in turn, can confound interpretations of drug responses and resistance development. Using BRAF-mutant melanoma cell lines as the prototype, we report on a joint theoretical and experimental investigation of the cell-state transition dynamics associated with BRAF inhibitor drug tolerance. Thermodynamically motivated surprisal analysis of transcriptome data was used to treat the cell population as an entropy maximizing system under the influence of time-dependent constraints. This permits the extraction of an epigenetic potential landscape for drug-induced phenotypic evolution. Single-cell flow cytometry data of the same system were modeled with a modified Fokker-Planck-type kinetic model. The two approaches yield a consistent picture that accounts for the phenotypic heterogeneity observed over the course of drug tolerance development. The results reveal that, in certain plastic cancers, the population heterogeneity and evolution of cell phenotypes may be understood by accounting for the competing interactions of the epigenetic potential landscape and state-dependent cell proliferation. Accounting for such competition permits accurate, experimentally verifiable predictions that can potentially guide the design of effective treatment strategies.
Mesh-Begriff(e)	Antineoplastic Agents/pharmacology ; Cell Line, Tumor ; Cell Proliferation/drug effects ; Cell Proliferation/genetics ; Drug Resistance, Neoplasm/drug effects ; Drug Resistance, Neoplasm/genetics ; Evolution, Molecular ; Humans ; Melanoma/genetics ; Melanoma/physiopathology ; Models, Biological ; Phenotype ; Transcriptome/drug effects ; Transcriptome/genetics
Chemische Substanzen	Antineoplastic Agents
Sprache	Englisch
Erscheinungsdatum	2019-06-05
Erscheinungsland	United States
Dokumenttyp	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, N.I.H., Intramural
ZDB-ID	2193340-6
ISSN	1553-7358 ; 1553-734X
ISSN (online)	1553-7358
ISSN	1553-734X
DOI	10.1371/journal.pcbi.1007034
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

Zusatzmaterialien

Über subito bestellen

Details ▾
- Kostenpflichtig bestellen

Artikel ; Online: Raman-guided subcellular pharmaco-metabolomics for metastatic melanoma cells.

Du, Jiajun / Su, Yapeng / Qian, Chenxi / Yuan, Dan / Miao, Kun / Lee, Dongkwan / Ng, Alphonsus H C / Wijker, Reto S / Ribas, Antoni / Levine, Raphael D / Heath, James R / Wei, Lu

Nature communications

2020 Band 11, Heft 1, Seite(n) 4830

Abstract: Non-invasively probing metabolites within single live cells is highly desired but challenging. Here we utilize Raman spectro-microscopy for spatial mapping of metabolites within single cells, with the specific goal of identifying druggable metabolic ... ...

Abstract	Non-invasively probing metabolites within single live cells is highly desired but challenging. Here we utilize Raman spectro-microscopy for spatial mapping of metabolites within single cells, with the specific goal of identifying druggable metabolic susceptibilities from a series of patient-derived melanoma cell lines. Each cell line represents a different characteristic level of cancer cell de-differentiation. First, with Raman spectroscopy, followed by stimulated Raman scattering (SRS) microscopy and transcriptomics analysis, we identify the fatty acid synthesis pathway as a druggable susceptibility for differentiated melanocytic cells. We then utilize hyperspectral-SRS imaging of intracellular lipid droplets to identify a previously unknown susceptibility of lipid mono-unsaturation within de-differentiated mesenchymal cells with innate resistance to BRAF inhibition. Drugging this target leads to cellular apoptosis accompanied by the formation of phase-separated intracellular membrane domains. The integration of subcellular Raman spectro-microscopy with lipidomics and transcriptomics suggests possible lipid regulatory mechanisms underlying this pharmacological treatment. Our method should provide a general approach in spatially-resolved single cell metabolomics studies.
Mesh-Begriff(e)	Apoptosis ; Cell Line, Tumor ; Fatty Acids/metabolism ; Humans ; Lipid Droplets ; Lipid Metabolism ; Lipidomics ; Lipids ; Melanoma/metabolism ; Metabolomics/methods ; Microscopy/methods ; Oleic Acid ; Spectrum Analysis, Raman/methods ; Stearoyl-CoA Desaturase/metabolism ; Transcriptome
Chemische Substanzen	Fatty Acids ; Lipids ; Oleic Acid (2UMI9U37CP) ; SCD1 protein, human (EC 1.14.19.1) ; Stearoyl-CoA Desaturase (EC 1.14.19.1)
Sprache	Englisch
Erscheinungsdatum	2020-09-24
Erscheinungsland	England
Dokumenttyp	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2553671-0
ISSN	2041-1723 ; 2041-1723
ISSN (online)	2041-1723
ISSN	2041-1723
DOI	10.1038/s41467-020-18376-x
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

Zusatzmaterialien

Über subito bestellen

Details ▾
- Kostenpflichtig bestellen

Artikel ; Online: An inkjet printed, roll-coated digital microfluidic device for inexpensive, miniaturized diagnostic assays.

Dixon, Christopher / Ng, Alphonsus H C / Fobel, Ryan / Miltenburg, Mark B / Wheeler, Aaron R

Lab on a chip

2016 Band 16, Heft 23, Seite(n) 4560–4568

Abstract: The diagnosis of infectious disease is typically carried out at the point-of-care (POC) using the lateral flow assay (LFA). While cost-effective and portable, LFAs often lack the clinical sensitivity and specificity required for accurate diagnoses. In ... ...

Abstract	The diagnosis of infectious disease is typically carried out at the point-of-care (POC) using the lateral flow assay (LFA). While cost-effective and portable, LFAs often lack the clinical sensitivity and specificity required for accurate diagnoses. In response to this challenge, we introduce a new digital microfluidic (DMF) platform fabricated using a custom inkjet printing and roll-coating process that is scalable to mass production. The performance of the new devices is on par with that of traditional DMF devices fabricated in a cleanroom, with a materials cost for the new devices of only US $0.63 per device. To evaluate the usefulness of the new platform, we performed a 13-step rubella virus (RV) IgG immunoassay on the inkjet printed, roll-coated devices, which yielded a limit of detection of 0.02 IU mL
Sprache	Englisch
Erscheinungsdatum	2016-11-15
Erscheinungsland	England
Dokumenttyp	Journal Article
ZDB-ID	2056646-3
ISSN	1473-0189 ; 1473-0197
ISSN (online)	1473-0189
ISSN	1473-0197
DOI	10.1039/c6lc01064d
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

Zusatzmaterialien

Über subito bestellen

Details ▾
- Kostenpflichtig bestellen

Zum Seitenanfang

Zusatzmaterialien

Kategorien

Verfügbar in ZB MED Köln/Königswinter

Über subito bestellen

Zusatzmaterialien

Kategorien

Über subito bestellen

Fernleihe an ZB MED

Volltext online

Zusatzmaterialien

Kategorien

Fernleihe an ZB MED

Zusatzmaterialien

Kategorien

Über subito bestellen

Volltext online

Zusatzmaterialien

Kategorien

Fernleihe an ZB MED

Volltext online

Zusatzmaterialien

Kategorien

Fernleihe an ZB MED

Zusatzmaterialien

Kategorien

Über subito bestellen

Zusatzmaterialien

Kategorien

Über subito bestellen

Zusatzmaterialien

Kategorien

Über subito bestellen

Zusatzmaterialien

Kategorien

Über subito bestellen