Artikel: Single-Stranded DNA Cleavage by Divergent CRISPR-Cas9 Enzymes
Molecular cell. 2015 Nov. 05, v. 60
2015
Abstract: Double-stranded DNA (dsDNA) cleavage by Cas9 is a hallmark of type II CRISPR-Cas immune systems. Cas9-guide RNA complexes recognize 20-base-pair sequences in DNA and generate a site-specific double-strand break, a robust activity harnessed for genome ... ...
Abstract | Double-stranded DNA (dsDNA) cleavage by Cas9 is a hallmark of type II CRISPR-Cas immune systems. Cas9-guide RNA complexes recognize 20-base-pair sequences in DNA and generate a site-specific double-strand break, a robust activity harnessed for genome editing. DNA recognition by all studied Cas9 enzymes requires a protospacer adjacent motif (PAM) next to the target site. We show that Cas9 enzymes from evolutionarily divergent bacteria can recognize and cleave single-stranded DNA (ssDNA) by an RNA-guided, PAM-independent recognition mechanism. Comparative analysis shows that in contrast to the type II-A S. pyogenes Cas9 that is widely used for genome engineering, the smaller type II-C Cas9 proteins have limited dsDNA binding and unwinding activity and promiscuous guide RNA specificity. These results indicate that inefficiency of type II-C Cas9 enzymes for genome editing results from a limited ability to cleave dsDNA and suggest that ssDNA cleavage was an ancestral function of the Cas9 enzyme family. |
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Schlagwörter | bacteria ; DNA damage ; enzymes ; genetic engineering ; genome ; immune system ; nucleotide sequences ; proteins ; RNA ; single-stranded DNA |
Sprache | Englisch |
Erscheinungsverlauf | 2015-1105 |
Umfang | p. 398-407. |
Erscheinungsort | Elsevier Inc. |
Dokumenttyp | Artikel |
ZDB-ID | 1415236-8 |
ISSN | 1097-4164 ; 1097-2765 |
ISSN (online) | 1097-4164 |
ISSN | 1097-2765 |
DOI | 10.1016/j.molcel.2015.10.030 |
Datenquelle | NAL Katalog (AGRICOLA) |
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