LIVIVO - Das Suchportal für Lebenswissenschaften

switch to English language
Erweiterte Suche

Suchergebnis

Treffer 1 - 10 von insgesamt 81530

Suchoptionen

  1. Artikel ; Online: Meet the author: Ling Wang.

    Chakraborty, Sonhita / Wang, Ling

    Molecular cell

    2023  Band 83, Heft 7, Seite(n) 1013–1015

    Abstract: Ling Wang, first author of "Head-on and co-directional RNA polymerase collisions orchestrate ...

    Abstract Ling Wang, first author of "Head-on and co-directional RNA polymerase collisions orchestrate bidirectional transcription termination" in this issue of Molecular Cell, speaks about her motivations for becoming a scientist, the challenges she faced during the pandemic, and her pedagogy as a new principal investigator.
    Mesh-Begriff(e) Female ; Humans ; DNA-Directed RNA Polymerases ; Transcription, Genetic
    Chemische Substanzen DNA-Directed RNA Polymerases (EC 2.7.7.6)
    Sprache Englisch
    Erscheinungsdatum 2023-03-17
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 1415236-8
    ISSN 1097-4164 ; 1097-2765
    ISSN (online) 1097-4164
    ISSN 1097-2765
    DOI 10.1016/j.molcel.2023.03.007
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Volltext online

    Zusatzmaterialien

    Kategorien

    Verfügbar in ZB MED Köln/Königswinter

    Zs.A 5055: Hefte anzeigen Standort:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  2. Artikel ; Online: Meet the author: Ling Wang

    Wang, Ling

    Molecular Cell. 2023 Apr., v. 83, no. 7 p.1013-1015

    2023  

    Abstract: Ling Wang, first author of “Head-on and co-directional RNA polymerase collisions orchestrate ...

    Abstract Ling Wang, first author of “Head-on and co-directional RNA polymerase collisions orchestrate bidirectional transcription termination” in this issue of Molecular Cell, speaks about her motivations for becoming a scientist, the challenges she faced during the pandemic, and her pedagogy as a new principal investigator.
    Schlagwörter DNA-directed RNA polymerase ; pandemic ; scientists ; transcription termination
    Sprache Englisch
    Erscheinungsverlauf 2023-04
    Umfang p. 1013-1015.
    Erscheinungsort Elsevier Inc.
    Dokumenttyp Artikel ; Online
    ZDB-ID 1415236-8
    ISSN 1097-4164 ; 1097-2765
    ISSN (online) 1097-4164
    ISSN 1097-2765
    DOI 10.1016/j.molcel.2023.03.007
    Datenquelle NAL Katalog (AGRICOLA)

    Volltext online

    Zusatzmaterialien

    Kategorien

    Verfügbar in ZB MED Bonn

    Z 2005/501: Hefte anzeigen

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

    Fernleihe an ZB MED

    Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

  3. Artikel ; Online: Ling-Gui-Zhu-Gan decoction protects against doxorubicin-induced myocardial injury by downregulating ferroptosis.

    Yang, Ya-Li / Zhao, Chuan-Zhi / Zhao, Chun-Chun / Wen, Zhong-Yu / Ma, Yao-Yao / Zhao, Xiao-Ni / Wang, Liang / Huang, Jin-Ling / Zhou, Peng

    The Journal of pharmacy and pharmacology

    2024  Band 76, Heft 4, Seite(n) 405–415

    Abstract: Objective: To investigate the mechanism of Ling-Gui-Zhu-Gan decoction (LGZGD) protects against ...

    Abstract Objective: To investigate the mechanism of Ling-Gui-Zhu-Gan decoction (LGZGD) protects against doxorubicin (DOX)-induced myocardial injury.
    Methods: In vivo experiment, rats were divided into six groups: normal group, model group (15 mg/kg, DOX), Dex group(150 mg/kg, Dex), LGZGD-L group (2.1 g/kg), LGZGD-M group (4.2 g/kg), and LGZGD-H group (8.4 g/kg). We used HE and Masson staining to observe the histopathological changes, echocardiography to assess the cardiac function, and western blot and RT-qPCR to detect the expressions of Nrf2, GPX4, Fpn1, and Ptgs2. In vitro experiment, we used immunofluorescence to detect ROS production, and RT-qPCR to detect gene expression of GPX4, Fpn1, and Ptgs2.
    Key findings: In vivo, LGZGD improved cardiac systolic function. LGZGD significantly reduced MDA, LDH, and CK levels, increased SOD activity, enhanced the protein expression of Nrf2, GPX4, and Fpn1, and decreased Ptgs2 levels. In vitro, LGZGD-containing serum significantly reduced ROS, increased the gene expression of GPX4 and Fpn1, and decreased the gene expression of Ptgs2. Furthermore, compared with the LGZGD (si-NC) group, the LGZGD (si-Nrf2) group had decreased gene expression of Nrf2, GPX4, and Fpn1 and increased gene expression of Ptgs2.
    Conclusions: LGZGD can ameliorate DOX-cardiotoxicity by activating the Nrf2 signaling pathway and inhibiting ferroptosis in cardiomyocytes.
    Mesh-Begriff(e) Rats ; Animals ; Ferroptosis ; Cyclooxygenase 2 ; NF-E2-Related Factor 2 ; Reactive Oxygen Species ; Doxorubicin/toxicity ; Plant Extracts
    Chemische Substanzen ling-gui-zhu-gan decoction ; Cyclooxygenase 2 (EC 1.14.99.1) ; NF-E2-Related Factor 2 ; Reactive Oxygen Species ; Doxorubicin (80168379AG) ; Plant Extracts
    Sprache Englisch
    Erscheinungsdatum 2024-01-19
    Erscheinungsland England
    Dokumenttyp Journal Article
    ZDB-ID 3107-0
    ISSN 2042-7158 ; 0022-3573 ; 0373-1022
    ISSN (online) 2042-7158
    ISSN 0022-3573 ; 0373-1022
    DOI 10.1093/jpp/rgae005
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Zusatzmaterialien

    Kategorien

    Verfügbar in ZB MED Köln/Königswinter

    Uf I Zs.149: Hefte anzeigen Standort:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  4. Artikel: Targeting the PANoptosis signaling pathway for myocardial protection: therapeutic potential of Xian Ling Gu Bao capsule.

    Wu, Xiaoyu / Wei, Jiajia / Zhang, Wenfang / Yi, Yang / Wang, Tingting / Gong, Qihai / Liu, Xin / Li, Haibo / Gao, Jianmei

    Frontiers in pharmacology

    2024  Band 15, Seite(n) 1391511

    Abstract: Introduction: ...

    Abstract Introduction:
    Sprache Englisch
    Erscheinungsdatum 2024-05-10
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2587355-6
    ISSN 1663-9812
    ISSN 1663-9812
    DOI 10.3389/fphar.2024.1391511
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Zusatzmaterialien

    Kategorien

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  5. Artikel ; Online: Rapid classification and identification of chemical compositions of Pu-zhi-hui-ling decoction by UHPLC-Q-Orbitrap HRMS.

    Shao, Jia / Zheng, Yanxue / Wang, Yuanyuan / Li, Guohui / Wei, Jinxia / Cheng, Wenbo / Li, Yubo

    Natural product research

    2024  , Seite(n) 1–10

    Abstract: Pu-zhi-hui-ling decoction (PZHLD) is a traditional Chinese medicine (TCM) formula for the treatment ...

    Abstract Pu-zhi-hui-ling decoction (PZHLD) is a traditional Chinese medicine (TCM) formula for the treatment of Alzheimer's disease (AD), but its chemical composition has not been reported. In this study, we aimed to establish a mass spectrometry (MS) analysis method for rapid classification and identification of the chemical constituents in PZHLD. The sample was analysed by ultrahigh-performance liquid chromatography coupled to quadrupole Orbitrap high-resolution mass spectrometry (UHPLC-Q-Orbitrap HRMS). The chemical constituents of PZHLD were identified based on accurate MS data, fragmentation characteristics of MS/MS, and reference information described in the literature. A total of 123 chemical constituents were identified. In addition, we summarised the fragmentation pathways of the chemical constituents in PZHLD. Our finding might lay the foundation for the further pharmacodynamic study and clinical application of PZHLD.
    Sprache Englisch
    Erscheinungsdatum 2024-01-03
    Erscheinungsland England
    Dokumenttyp Journal Article
    ZDB-ID 2185747-7
    ISSN 1478-6427 ; 1478-6419
    ISSN (online) 1478-6427
    ISSN 1478-6419
    DOI 10.1080/14786419.2023.2299302
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Zusatzmaterialien

    Kategorien

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  6. Artikel ; Online: Ling-Gui-Zhu-Gan decoction ameliorates nonalcoholic fatty liver disease via modulating the gut microbiota.

    Chen, Lu-Ping / Zhang, Lin-Fang / Liu, Shuang / Hua, Hua / Zhang, Lei / Liu, Bao-Cheng / Wang, Rui-Rui

    Microbiology spectrum

    2024  Band 12, Heft 6, Seite(n) e0197923

    Abstract: ... with gut microbiota dysbiosis. Ling-Gui-Zhu-Gan decoction (LG) has been clinically used to treat NAFLD ... Importance: Until now, there has still been no study on the gut microbiota and metabolomics of Ling-Gui-Zhu ...

    Abstract Numerous studies have supported that nonalcoholic fatty liver disease (NAFLD) is highly associated with gut microbiota dysbiosis. Ling-Gui-Zhu-Gan decoction (LG) has been clinically used to treat NAFLD, but the underlying mechanism remains unknown. This study investigated the therapeutic effect and mechanisms of LG in mice with NAFLD induced by a high-fat diet (HD). An HD-induced NAFLD mice model was established to evaluate the efficacy of LG followed by biochemical and histopathological analysis. Metagenomics, metabolomics, and transcriptomics were used to explore the structure and metabolism of the gut microbiota. LG significantly improved hepatic function and decreased lipid droplet accumulation in HD-induced NAFLD mice. LG reversed the structure of the gut microbiota that is damaged by HD and improved intestinal barrier function. Meanwhile, the LG group showed a lower total blood bile acids (BAs) concentration, a shifted BAs composition, and a higher fecal short-chain fatty acids (SCFAs) concentration. Furthermore, LG could regulate the hepatic expression of genes associated with the primary BAs biosynthesis pathway and peroxisome proliferator-activated receptor (PPAR) signaling pathway. Our study suggested that LG could ameliorate NAFLD by altering the structure and metabolism of gut microbiota, while BAs and SCFAs are considered possible mediating substances.
    Importance: Until now, there has still been no study on the gut microbiota and metabolomics of Ling-Gui-Zhu-Gan decoction (LG) in nonalcoholic fatty liver disease (NAFLD) mouse models. Our study is the first to report on the reshaping of the structure and metabolism of the gut microbiota by LG, as well as explore the potential mechanism underlying the improvement of NAFLD. Specifically, our study demonstrates the potential of gut microbial-derived short-chain fatty acids (SCFAs) and blood bile acids (BAs) as mediators of LG therapy for NAFLD in animal models. Based on the results of transcriptomics, we further verified that LG attenuates NAFLD by restoring the metabolic disorder of BAs via the up-regulation of Fgf15/FXR in the ileum and down-regulation of CYP7A1/FXR in the liver. LG also reduces lipogenesis in NAFLD mice by mediating the peroxisome proliferator-activated receptor (PPAR) signaling pathway, which then contributes to reducing hepatic inflammation and improving intestinal barrier function to treat NAFLD.
    Mesh-Begriff(e) Animals ; Non-alcoholic Fatty Liver Disease/drug therapy ; Non-alcoholic Fatty Liver Disease/metabolism ; Gastrointestinal Microbiome/drug effects ; Drugs, Chinese Herbal/pharmacology ; Drugs, Chinese Herbal/therapeutic use ; Mice ; Male ; Disease Models, Animal ; Diet, High-Fat/adverse effects ; Dysbiosis/drug therapy ; Dysbiosis/microbiology ; Liver/drug effects ; Liver/metabolism ; Mice, Inbred C57BL ; Bile Acids and Salts/metabolism ; Fatty Acids, Volatile/metabolism ; Lipid Metabolism/drug effects ; Signal Transduction/drug effects ; Plant Extracts
    Chemische Substanzen Drugs, Chinese Herbal ; ling-gui-zhu-gan decoction ; Bile Acids and Salts ; Fatty Acids, Volatile ; Plant Extracts
    Sprache Englisch
    Erscheinungsdatum 2024-04-22
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 2807133-5
    ISSN 2165-0497 ; 2165-0497
    ISSN (online) 2165-0497
    ISSN 2165-0497
    DOI 10.1128/spectrum.01979-23
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Zusatzmaterialien

    Kategorien

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  7. Artikel: Exploring the Mechanism of Ling-Gui-Zhu-Gan Decoction in Ventricular Remodeling after Acute Myocardial Infarction Based on UPLC and

    Zhou, Peng / Zhang, Meng / Zhao, Xiao-Ni / Tang, Tong-Juan / Wang, Xiang / Huang, Lu-Lu / Kong, Qi / Wang, Liang / Huang, Jin-Ling

    Evidence-based complementary and alternative medicine : eCAM

    2022  Band 2022, Seite(n) 8593176

    Abstract: ... pathophysiological basis for the development of chronic heart failure (CHF). At present, Ling-Gui-Zhu-Gan decoction ...

    Abstract Ventricular remodeling (VR) after acute myocardial infarction (AMI) is an important pathophysiological basis for the development of chronic heart failure (CHF). At present, Ling-Gui-Zhu-Gan decoction (LGZGD) has been widely reported in the clinical treatment and basic research of cardiovascular diseases (CVDs), such as myocardial infarction, heart failure, and angina pectoris. However, the mechanism of LGZGD against VR after AMI remains unclear. Ultra-performance liquid chromatography (UPLC) was applied to investigate the major constituents of LGZGD, and molecular docking was used to predict the targets on the NLRP3/Caspase-1/GSDMD signaling pathway.
    Sprache Englisch
    Erscheinungsdatum 2022-05-16
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 2171158-6
    ISSN 1741-4288 ; 1741-427X
    ISSN (online) 1741-4288
    ISSN 1741-427X
    DOI 10.1155/2022/8593176
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Zusatzmaterialien

    Kategorien

    Verfügbar in ZB MED Köln/Königswinter

    Zs.A 5995: Hefte anzeigen Standort:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  8. Artikel ; Online: Successful treatment of immune-related cystitis by Chai-Ling-Tang (Sairei-To) in a gastric carcinoma patient: Case report and literature review.

    Wang, Zhiqiang / Zhu, Liping / Huang, Yong / Peng, Ling

    Explore (New York, N.Y.)

    2022  Band 19, Heft 3, Seite(n) 458–462

    Abstract: ... Oral administration of Chai-Ling-Tang (Sairei-To) alleviated lower urinary tract symptoms (LUTS). Histological ... The dose of Chai-Ling-Tang was maintained and stopped 2 months later without recurrence of LUTS ... shows that Chai-Ling-Tang is safe and effective for treating immune-related cystitis. The detailed ...

    Abstract Background: Immune checkpoint inhibitors (ICIs) have changed the landscape of advanced cancer treatment. However, immune checkpoint inhibitors can trigger effector T cells against self-antigens as well as tumor antigens, resulting in immune-related toxicities in normal organs, referred to as immune-related adverse events (irAEs).
    Case summary: A 56-year-old man with undifferentiated gastric carcinoma received sintilimab plus paclitaxel and tegafur therapy. After five cycles of treatment, the patient was referred to the hospital for sudden onset urinary frequency, micturition pain, and urinary incontinence. Cystoscopy revealed the entire bladder mucosa was red and edematous but there was no evidence of tumor. Oral administration of Chai-Ling-Tang (Sairei-To) alleviated lower urinary tract symptoms (LUTS). Histological analysis revealed numerous infiltrates of CD3-positive and CD8-positive cells into the urothelium but no atypia, indicating a diagnosis of immune-related cystitis. Interestingly, the urothelial epithelium infiltrated by lymphocytes and subepithelial inflammatory cells strongly expressed cell boundary PD-L1. The dose of Chai-Ling-Tang was maintained and stopped 2 months later without recurrence of LUTS. Since recovering from cystitis, the patient remains alive with no disease progression.
    Conclusion: This report shows that Chai-Ling-Tang is safe and effective for treating immune-related cystitis. The detailed mechanism of action requires further investigation.
    Mesh-Begriff(e) Male ; Humans ; Middle Aged ; Immune Checkpoint Inhibitors ; Drugs, Chinese Herbal/therapeutic use ; Autoimmune Diseases ; Cystitis/drug therapy ; Carcinoma/drug therapy
    Chemische Substanzen sairei-to ; Immune Checkpoint Inhibitors ; Drugs, Chinese Herbal
    Sprache Englisch
    Erscheinungsdatum 2022-04-04
    Erscheinungsland United States
    Dokumenttyp Review ; Case Reports ; Journal Article
    ZDB-ID 2183945-1
    ISSN 1878-7541 ; 1550-8307
    ISSN (online) 1878-7541
    ISSN 1550-8307
    DOI 10.1016/j.explore.2022.04.002
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Volltext online

    Zusatzmaterialien

    Kategorien

    Verfügbar in ZB MED Köln/Königswinter

    Zs.A 6635: Hefte anzeigen Standort:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  9. Artikel ; Online: Tone evaluation of Ling sound test in Mandarin tone version.

    Zuo, Yufeng / Wang, Yuxin / Liu, Shu / Yan, Hongxu / Liu, Lin / Wang, Xing

    Technology and health care : official journal of the European Society for Engineering and Medicine

    2022  Band 31, Heft 2, Seite(n) 607–620

    Abstract: Background: The Ling sound test cannot provide the test of Chinese tone for preschool children ... of the Ling sound test and four Chinese tones to evaluate the hearing level of Chinese hearing-impaired ... statistically analyzed to verify the validity of the Ling sound test in the Chinese tone version. In addition, this paper ...

    Abstract Background: The Ling sound test cannot provide the test of Chinese tone for preschool children with hearing aid and cochlear implants.
    Objective: The paper tries to design a new tone test method composed of the Ling sound test and four Chinese tones to evaluate the hearing level of Chinese hearing-impaired children.
    Methods: The tone identification rates of 20 cochlear implant children were statistically analyzed to verify the validity of the Ling sound test in the Chinese tone version. In addition, this paper analyzed the pronunciation characteristics of the Ling sound test in the Chongqing-accented Mandarin version of 20 subjects.
    Results: The identification rate of Ling six sounds was more than 97.0%, the identification rate of tone was more than 81.0%, and the identification rate of vowels was 83.1%, which was higher than that of consonants 79.0%. The Ling sound test n the Chongqing-accented Mandarin version has a narrower frequency range.
    Conclusion: The results verify the effectiveness and feasibility of the Ling sound test in the Chinese tone version in the assessment of frequency range and tone identification for cochlear implant users.
    Mesh-Begriff(e) Child, Preschool ; Humans ; Deafness/rehabilitation ; Speech Perception ; Language ; Cochlear Implants ; Hearing Aids
    Sprache Englisch
    Erscheinungsdatum 2022-09-25
    Erscheinungsland Netherlands
    Dokumenttyp Journal Article
    ZDB-ID 1159961-3
    ISSN 1878-7401 ; 0928-7329
    ISSN (online) 1878-7401
    ISSN 0928-7329
    DOI 10.3233/THC-220189
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Zusatzmaterialien

    Kategorien

    Verfügbar in ZB MED Köln/Königswinter

    Zs.A 3846: Hefte anzeigen Standort:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  10. Artikel ; Online: Network pharmacology and experimental validation to investigate the mechanism of Nao-Ling-Su capsule in the treatment of ischemia/reperfusion-induced acute kidney injury.

    Lin, Yongqiang / Xu, Lili / Lin, Huibin / Cui, Weiliang / Jiao, Yang / Wang, Bing / Li, Huifen / Wang, Xiaojie / Wu, Jichao

    Journal of ethnopharmacology

    2024  Band 326, Seite(n) 117958

    Abstract: Ethnopharmacological relevance: Nao-Ling-Su Capsule (NLSC) is a traditional prescription, which is ...

    Abstract Ethnopharmacological relevance: Nao-Ling-Su Capsule (NLSC) is a traditional prescription, which is composed of fifteen herbs such as epimedium, Polygala tenuifolia, and Schisandra chinensis. It has the effect of strengthening the brain, calming nerves, and protecting the kidney, which has been used clinically for many years to strengthen the brain and kidney. However, the effect of NLSC in the treatment of acute kidney injury (AKI) is still unclear.
    Aim of the study: The present study aims to elucidate the pharmacological actions of NLSC in the treatment of AKI.
    Materials and methods: Molecular targets for NLSC and AKI were obtained from various databases, and then we built networks of interactions between proteins (PPI) by employing string databases. Additionally, we employed the DAVID database to conduct gene ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses. Molecular docking was conducted to analyze the interaction between core components and their corresponding core targets. Next, the C57BL male mice model of ischemia/reperfusion damage (IRI) was developed, and the nephridial protective effect of NLSC was evaluated. The accuracy of the expected targets was confirmed using real-time quantitative polymerase chain reaction (RT-qPCR). The renal protective effect of NLSC was assessed using an immortalized human kidney tubular (HK-2) cell culture produced by oxygen-glucose deprivation (OGD).
    Results: Network pharmacology analysis identified 199 common targets from NLSC and AKI. STAT3, HSP90AA1, TP53, MAPK3, JUN, JAK2, and VEGFA could serve as potential drug targets and were associated with JAK2/STAT3 signaling pathway, PI3K-Akt signaling pathway, etc. The molecular docking analysis confirmed significant docking activity between the main bioactive components and core targets, including STAT3 and KIM-1. Moreover, the AKI mice model was successfully established and NLSC pretreatment could improve renal function and alleviate renal damage. NLSC could alleviate renal inflammation and tubular cell apoptosis, and decrease the expression of STAT3 and KIM-1 in AKI mice. In vitro, both NLSC and drug-containing serum may protect HK-2 cells by inhibiting STAT3 signaling, especially STAT3-mediated apoptosis and KIM-1 expression.
    Conclusion: NLSC could alleviate renal inflammation and apoptosis, exerting its beneficial effects by targeting the STAT3/KIM-1 pathway. NLSC is a promising candidate for AKI treatment and provides a new idea and method for the treatment of AKI.
    Mesh-Begriff(e) Humans ; Male ; Animals ; Mice ; Mice, Inbred C57BL ; Molecular Docking Simulation ; Network Pharmacology ; Phosphatidylinositol 3-Kinases ; Kidney ; Acute Kidney Injury/drug therapy ; Reperfusion Injury/drug therapy ; Ischemia ; Reperfusion ; Nephritis ; Inflammation ; Drugs, Chinese Herbal/pharmacology ; Drugs, Chinese Herbal/therapeutic use
    Chemische Substanzen Phosphatidylinositol 3-Kinases (EC 2.7.1.-) ; Drugs, Chinese Herbal
    Sprache Englisch
    Erscheinungsdatum 2024-02-22
    Erscheinungsland Ireland
    Dokumenttyp Journal Article
    ZDB-ID 134511-4
    ISSN 1872-7573 ; 0378-8741
    ISSN (online) 1872-7573
    ISSN 0378-8741
    DOI 10.1016/j.jep.2024.117958
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Volltext online

    Zusatzmaterialien

    Kategorien

    Verfügbar in ZB MED Köln/Königswinter

    Zs.A 1494: Hefte anzeigen Standort:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

Zum Seitenanfang