Artikel ; Online: Molecular authentication of commercial "Qian-hu" through the integration of nrDNA internal transcribed spacer 2 and nucleotide signature.
2024 Band 51, Heft 1, Seite(n) 639
Abstract: Background: Peucedani Radix, also known as "Qian-hu" is a traditional Chinese medicine derived ... Qian-hu" samples from various Chinese medicinal markets and 44 species representing genuine ... of the commercial "Qian-hu". The nucleotide signature specific to Peucedani Radix was subsequently developed ...
| Abstract | Background: Peucedani Radix, also known as "Qian-hu" is a traditional Chinese medicine derived from Peucedanum praeruptorum Dunn. It is widely utilized for treating wind-heat colds and coughs accompanied by excessive phlegm. However, due to morphological similarities, limited resources, and heightened market demand, numerous substitutes and adulterants of Peucedani Radix have emerged within the herbal medicine market. Moreover, Peucedani Radix is typically dried and sliced for sale, rendering traditional identification methods challenging. Materials and methods: We initially examined and compared 104 commercial "Qian-hu" samples from various Chinese medicinal markets and 44 species representing genuine, adulterants or substitutes, utilizing the mini barcode ITS2 region to elucidate the botanical origins of the commercial "Qian-hu". The nucleotide signature specific to Peucedani Radix was subsequently developed by analyzing the polymorphic sites within the aligned ITS2 sequences. Results: The results demonstrated a success rate of 100% and 93.3% for DNA extraction and PCR amplification, respectively. Forty-five samples were authentic "Qian-hu", while the remaining samples were all adulterants, originating from nine distinct species. Peucedani Radix, its substitutes, and adulterants were successfully identified based on the neighbor-joining tree. The 24-bp nucleotide signature (5'-ATTGTCGTACGAATCCTCGTCGTC-3') revealed distinct differences between Peucedani Radix and its common substitutes and adulterants. The newly designed specific primers (PR-F/PR-R) can amplify the nucleotide signature region from commercial samples and processed materials with severe DNA degradation. Conclusions: We advocate for the utilization of ITS2 and nucleotide signature for the rapid and precise identification of herbal medicines and their adulterants to regulate the Chinese herbal medicine industry. |
|---|---|
| Mesh-Begriff(e) | DNA, Plant/genetics ; DNA Barcoding, Taxonomic/methods ; Drugs, Chinese Herbal/standards ; Apiaceae/genetics ; Apiaceae/classification ; Medicine, Chinese Traditional/standards ; DNA, Ribosomal Spacer/genetics ; Drug Contamination ; Plants, Medicinal/genetics ; Phylogeny ; Sequence Analysis, DNA/methods ; Polymerase Chain Reaction/methods ; Nucleotides/genetics ; Nucleotides/analysis |
| Chemische Substanzen | DNA, Plant ; Drugs, Chinese Herbal ; DNA, Ribosomal Spacer ; Nucleotides |
| Sprache | Englisch |
| Erscheinungsdatum | 2024-05-10 |
| Erscheinungsland | Netherlands |
| Dokumenttyp | Journal Article |
| ZDB-ID | 186544-4 |
| ISSN | 1573-4978 ; 0301-4851 |
| ISSN (online) | 1573-4978 |
| ISSN | 0301-4851 |
| DOI | 10.1007/s11033-024-09557-8 |
| Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
Volltext online
Zusatzmaterialien
Kategorien
Verfügbar in ZB MED Köln/Königswinter
| Zs.A 1140: Hefte anzeigen | Standort: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG) |
Über subito bestellen
Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.