LIVIVO - Das Suchportal für Lebenswissenschaften

switch to English language
Erweiterte Suche

Suchergebnis

Treffer 1 - 4 von insgesamt 4

Suchoptionen

  1. Artikel ; Online: Mitochondrial RNA granules are fluid condensates positioned by membrane dynamics.

    Rey, Timo / Zaganelli, Sofia / Cuillery, Emilie / Vartholomaiou, Evangelia / Croisier, Marie / Martinou, Jean-Claude / Manley, Suliana

    Nature cell biology

    2020  Band 22, Heft 10, Seite(n) 1180–1186

    Abstract: Mitochondria contain the genetic information and expression machinery to produce essential respiratory chain proteins. Within the mitochondrial matrix, newly synthesized RNA, RNA processing proteins and mitoribosome assembly factors form punctate sub- ... ...

    Abstract Mitochondria contain the genetic information and expression machinery to produce essential respiratory chain proteins. Within the mitochondrial matrix, newly synthesized RNA, RNA processing proteins and mitoribosome assembly factors form punctate sub-compartments referred to as mitochondrial RNA granules (MRGs)
    Mesh-Begriff(e) HeLa Cells ; Humans ; Microscopy, Fluorescence ; Mitochondria/physiology ; Mitochondrial Dynamics ; Mitochondrial Membranes/physiology ; Mitochondrial Proteins/genetics ; Mitochondrial Proteins/metabolism ; Mitochondrial Ribosomes/physiology ; RNA, Mitochondrial/genetics ; RNA, Mitochondrial/metabolism ; RNA-Binding Proteins/genetics ; RNA-Binding Proteins/metabolism
    Chemische Substanzen Mitochondrial Proteins ; RNA, Mitochondrial ; RNA-Binding Proteins
    Sprache Englisch
    Erscheinungsdatum 2020-09-28
    Erscheinungsland England
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1474722-4
    ISSN 1476-4679 ; 1465-7392
    ISSN (online) 1476-4679
    ISSN 1465-7392
    DOI 10.1038/s41556-020-00584-8
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Zusatzmaterialien

    Kategorien

    Verfügbar in ZB MED Köln/Königswinter

    Zs.A 5169: Hefte anzeigen Standort:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MG 12: Hefte anzeigen

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  2. Artikel ; Online: Distinct fission signatures predict mitochondrial degradation or biogenesis.

    Kleele, Tatjana / Rey, Timo / Winter, Julius / Zaganelli, Sofia / Mahecic, Dora / Perreten Lambert, Hélène / Ruberto, Francesco Paolo / Nemir, Mohamed / Wai, Timothy / Pedrazzini, Thierry / Manley, Suliana

    Nature

    2021  Band 593, Heft 7859, Seite(n) 435–439

    Abstract: Mitochondrial fission is a highly regulated process that, when disrupted, can alter metabolism, proliferation and ... ...

    Abstract Mitochondrial fission is a highly regulated process that, when disrupted, can alter metabolism, proliferation and apoptosis
    Mesh-Begriff(e) Actins ; Animals ; COS Cells ; Cell Survival ; Cells, Cultured ; Chlorocebus aethiops ; DNA, Mitochondrial/analysis ; DNA, Mitochondrial/metabolism ; Dynamins ; Endoplasmic Reticulum ; Humans ; Lysosomes ; Membrane Proteins ; Mice ; Mitochondria/genetics ; Mitochondria/metabolism ; Mitochondrial Dynamics ; Mitochondrial Proteins ; Mitophagy
    Chemische Substanzen Actins ; DNA, Mitochondrial ; FIS1 protein, human ; Membrane Proteins ; Mitochondrial Proteins ; DNM1L protein, human (EC 3.6.5.5) ; Dynamins (EC 3.6.5.5)
    Sprache Englisch
    Erscheinungsdatum 2021-05-05
    Erscheinungsland England
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 120714-3
    ISSN 1476-4687 ; 0028-0836
    ISSN (online) 1476-4687
    ISSN 0028-0836
    DOI 10.1038/s41586-021-03510-6
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Zusatzmaterialien

    Kategorien

    Verfügbar in ZB MED Köln/Königswinter

    Zs.A 26: Hefte anzeigen Standort:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MG 9: Hefte anzeigen
    Zs.MO 244: Hefte anzeigen

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  3. Artikel ; Online: FASTKD1 and FASTKD4 have opposite effects on expression of specific mitochondrial RNAs, depending upon their endonuclease-like RAP domain.

    Boehm, Erik / Zaganelli, Sofia / Maundrell, Kinsey / Jourdain, Alexis A / Thore, Stéphane / Martinou, Jean-Claude

    Nucleic acids research

    2017  Band 45, Heft 10, Seite(n) 6135–6146

    Abstract: FASTK family proteins have been identified as regulators of mitochondrial RNA homeostasis linked to mitochondrial diseases, but much remains unknown about these proteins. We show that CRISPR-mediated disruption of FASTKD1 increases ND3 mRNA level, while ... ...

    Abstract FASTK family proteins have been identified as regulators of mitochondrial RNA homeostasis linked to mitochondrial diseases, but much remains unknown about these proteins. We show that CRISPR-mediated disruption of FASTKD1 increases ND3 mRNA level, while disruption of FASTKD4 reduces the level of ND3 and of other mature mRNAs including ND5 and CYB, and causes accumulation of ND5-CYB precursor RNA. Disrupting both FASTKD1 and FASTKD4 in the same cell results in decreased ND3 mRNA similar to the effect of depleting FASTKD4 alone, indicating that FASTKD4 loss is epistatic. Interestingly, very low levels of FASTKD4 are sufficient to prevent ND3 loss and ND5-CYB precursor accumulation, suggesting that FASTKD4 may act catalytically. Furthermore, structural modeling predicts that each RAP domain of FASTK proteins contains a nuclease fold with a conserved aspartate residue at the putative active site. Accordingly, mutation of this residue in FASTKD4 abolishes its function. Experiments with FASTK chimeras indicate that the RAP domain is essential for the function of the FASTK proteins, while the region upstream determines RNA targeting and protein localization. In conclusion, this paper identifies new aspects of FASTK protein biology and suggests that the RAP domain function depends on an intrinsic nucleolytic activity.
    Mesh-Begriff(e) Amino Acid Sequence ; CRISPR-Cas Systems ; Cytochromes b/genetics ; Electron Transport Complex I/genetics ; Gene Expression Regulation ; Gene Knockdown Techniques ; HEK293 Cells ; Humans ; Mitochondria/metabolism ; Mitochondria/ultrastructure ; Mitochondrial Proteins/chemistry ; Mitochondrial Proteins/genetics ; Mitochondrial Proteins/physiology ; Models, Molecular ; Protein Conformation ; Protein Domains ; RNA/genetics ; RNA/metabolism ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; RNA, Mitochondrial ; RNA-Binding Proteins/chemistry ; RNA-Binding Proteins/genetics ; RNA-Binding Proteins/physiology ; Sequence Alignment ; Sequence Homology ; Transcription, Genetic
    Chemische Substanzen FASTKD1 protein, human ; Mitochondrial Proteins ; RNA, Messenger ; RNA, Mitochondrial ; RNA-Binding Proteins ; TBRG4 protein, human ; RNA (63231-63-0) ; Cytochromes b (9035-37-4) ; MT-ND5 protein, human (EC 1.6.99.3) ; Electron Transport Complex I (EC 7.1.1.2) ; MT-ND3 protein, human (EC 7.1.1.2)
    Sprache Englisch
    Erscheinungsdatum 2017-03-20
    Erscheinungsland England
    Dokumenttyp Comparative Study ; Journal Article
    ZDB-ID 186809-3
    ISSN 1362-4962 ; 1362-4954 ; 0301-5610 ; 0305-1048
    ISSN (online) 1362-4962 ; 1362-4954
    ISSN 0301-5610 ; 0305-1048
    DOI 10.1093/nar/gkx164
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Zusatzmaterialien

    Kategorien

    Verfügbar in ZB MED Köln/Königswinter

    Zs.A 1067: Hefte anzeigen Standort:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  4. Artikel ; Online: The Pseudouridine Synthase RPUSD4 Is an Essential Component of Mitochondrial RNA Granules.

    Zaganelli, Sofia / Rebelo-Guiomar, Pedro / Maundrell, Kinsey / Rozanska, Agata / Pierredon, Sandra / Powell, Christopher A / Jourdain, Alexis A / Hulo, Nicolas / Lightowlers, Robert N / Chrzanowska-Lightowlers, Zofia M / Minczuk, Michal / Martinou, Jean-Claude

    The Journal of biological chemistry

    2017  Band 292, Heft 11, Seite(n) 4519–4532

    Abstract: Mitochondrial gene expression is a fundamental process that is largely dependent on nuclear-encoded proteins. Several steps of mitochondrial RNA processing and maturation, including RNA post-transcriptional modification, appear to be spatially organized ... ...

    Abstract Mitochondrial gene expression is a fundamental process that is largely dependent on nuclear-encoded proteins. Several steps of mitochondrial RNA processing and maturation, including RNA post-transcriptional modification, appear to be spatially organized into distinct foci, which we have previously termed mitochondrial RNA granules (MRGs). Although an increasing number of proteins have been localized to MRGs, a comprehensive analysis of the proteome of these structures is still lacking. Here, we have applied a microscopy-based approach that has allowed us to identify novel components of the MRG proteome. Among these, we have focused our attention on RPUSD4, an uncharacterized mitochondrial putative pseudouridine synthase. We show that RPUSD4 depletion leads to a severe reduction of the steady-state level of the 16S mitochondrial (mt) rRNA with defects in the biogenesis of the mitoribosome large subunit and consequently in mitochondrial translation. We report that RPUSD4 binds 16S mt-rRNA, mt-tRNA
    Mesh-Begriff(e) Cell Line ; Humans ; Intramolecular Transferases/analysis ; Intramolecular Transferases/genetics ; Intramolecular Transferases/metabolism ; Mitochondria/genetics ; Mitochondria/metabolism ; Mitochondrial Proteins/metabolism ; Protein Transport ; RNA/metabolism ; RNA Interference ; RNA, Mitochondrial ; RNA, Ribosomal, 16S/metabolism ; RNA, Small Interfering/genetics ; RNA, Transfer, Met/metabolism ; RNA, Transfer, Phe/metabolism
    Chemische Substanzen Mitochondrial Proteins ; RNA, Mitochondrial ; RNA, Ribosomal, 16S ; RNA, Small Interfering ; RNA, Transfer, Met ; RNA, Transfer, Phe ; RNA (63231-63-0) ; Intramolecular Transferases (EC 5.4.-) ; pseudouridine synthases (EC 5.4.99.-)
    Sprache Englisch
    Erscheinungsdatum 2017-01-12
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 2997-x
    ISSN 1083-351X ; 0021-9258
    ISSN (online) 1083-351X
    ISSN 0021-9258
    DOI 10.1074/jbc.M116.771105
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Zusatzmaterialien

    Kategorien

    Verfügbar in ZB MED Köln/Königswinter

    Uc I Zs.108: Hefte anzeigen Standort:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

Zum Seitenanfang