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  1. Artikel: Isolation and characterization of a new agglutinin from the red marine alga Hypnea cervicornis J. Agardh.

    Nascimento, K S / Nagano, C S / Nunes, E V / Rodrigues, R F / Goersch, G V / Cavada, B S / Calvete, J J / Saker-Sampaio, S / Farias, W R L / Sampaio, A H

    Biochemistry and cell biology = Biochimie et biologie cellulaire

    2006  Band 84, Heft 1, Seite(n) 49–54

    Abstract: The biochemical characterization of a new lectin (Hypnea cervicornis agglutinin or HCA) isolated from the Brazilian red alga H. cervicornis is reported. The haemagglutinating activity of the lectin was only inhibited by the glycoprotein porcine stomach ... ...

    Abstract The biochemical characterization of a new lectin (Hypnea cervicornis agglutinin or HCA) isolated from the Brazilian red alga H. cervicornis is reported. The haemagglutinating activity of the lectin was only inhibited by the glycoprotein porcine stomach mucin at a minimum inhibitory concentration of 19 microg x mL(-1). No haemagglutination inhibition was detected after the addition of simple sugars. The MALDI-TOF molecular masses of native and reduced and carbamidomethylated HCA were, respectively, 9196.6 Da and 9988.2 Da, indicating that the primary structure of the protein is crosslinked by 7 disulfide bonds. This unusual structural feature among lectins, along with its N-terminal sequence and amino-acid composition, clearly shows that HCA belongs to a protein family distinct from the isolectins Hypnin A1 and A2 isolated from the related Japanese alga Hypnea japonica. On the other hand, HCA displayed a high degree of similarity to the agglutinin from the Brazilian species Hypnea musciformis. Our data indicate the occurrence of structural diversity among lectins of closely related species living in distant ecosystems, i.e., the Pacific coast of Japan and the Atlantic coast of Brazil, and support the hypothesis that the lectin content (lectinome) might serve as a biomarker for taxonomical purposes.
    Mesh-Begriff(e) Agglutinins/chemistry ; Agglutinins/isolation & purification ; Amino Acid Sequence ; Amino Acids ; Animals ; Chromatography, Ion Exchange ; Hemagglutination ; Hemagglutination Tests ; Molecular Sequence Data ; Rhodophyta/chemistry
    Chemische Substanzen Agglutinins ; Amino Acids
    Sprache Englisch
    Erscheinungsdatum 2006-02
    Erscheinungsland Canada
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 54104-7
    ISSN 0829-8211
    ISSN 0829-8211
    DOI 10.1139/o05-152
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel: Morboletalidad hospitalaria por tuberculosis. Observaciones del Hospital F. J. Muñiz en el período 1965-70.

    Leston, J M / Gonzalez Montaner, L J / Gonzalez Cambaceres, C E / Calvete, C

    Prensa medica argentina

    1971  Band 58, Heft 26, Seite(n) 1332–1338

    Titelübersetzung Hospital tuberculosis mortality. Cases in the F. J. Muñiz Hospital in the 1965-70 period.
    Mesh-Begriff(e) Adult ; Age Factors ; Aged ; Argentina ; Female ; Humans ; Male ; Middle Aged ; Time Factors ; Tuberculosis, Pulmonary/mortality
    Sprache Spanisch
    Erscheinungsdatum 1971-08
    Erscheinungsland Argentina
    Dokumenttyp Journal Article
    ZDB-ID 128734-5
    ISSN 0032-745X
    ISSN 0032-745X
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel ; Online: Journal of Proteomics turns 10: Happy anniversary! and beyond.

    Calvete, Juan J

    Journal of proteomics

    2019  Band 198, Seite(n) iii

    Mesh-Begriff(e) Anniversaries and Special Events ; History, 21st Century ; Humans ; Periodicals as Topic/history ; Proteomics
    Sprache Englisch
    Erscheinungsdatum 2019-03-07
    Erscheinungsland Netherlands
    Dokumenttyp Editorial ; Historical Article
    ZDB-ID 2400835-7
    ISSN 1876-7737 ; 1874-3919
    ISSN (online) 1876-7737
    ISSN 1874-3919
    DOI 10.1016/j.jprot.2019.03.002
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  4. Artikel ; Online: Snake venomics - from low-resolution toxin-pattern recognition to toxin-resolved venom proteomes with absolute quantification.

    Calvete, Juan J

    Expert review of proteomics

    2018  Band 15, Heft 7, Seite(n) 555–568

    Abstract: Introduction: Venoms are integrated phenotypes used by a wide range of organisms for predatory and defensive purposes. The study of venoms is of great interest in diverse fields, such as evolutionary ecology and biotechnology. Omics technologies have ... ...

    Abstract Introduction: Venoms are integrated phenotypes used by a wide range of organisms for predatory and defensive purposes. The study of venoms is of great interest in diverse fields, such as evolutionary ecology and biotechnology. Omics technologies have contributed to understanding the evolutionary mechanisms that molded snake venoms to their present-day structural and functional variability landscape. Areas covered: This review article reflects on two recent implementations in venomics: absolute quantification of intact proteins by elemental mass spectrometry, and top-down molecular mass spectrometry. Expert commentary: Leveraging on a new way of polyatomic interference removal, a triple quadrupole inductively coupled plasma mass spectrometry configuration has proven feasible for the absolute quantification of venom toxins via sulfur detection. A major advantage of this approach over quantitative molecular mass spectrometry techniques is that only a generic S-standard is required to quantify all the chromatographically separated sulfur-containing fractions. Top-down venomics is in its infancy but, due to recent hardware and software developments, is gaining momentum. Proteoform-resolved venom proteomes are needed to understand the spatio-temporal variability landscape underlying the adaptations that drive intraspecific venom evolution. Integrating top-down venomics and absolute proteoform quantification into a novel elemental and molecular mass spectrometry configuration will represent a quantitative leap in the study of individual venoms.
    Mesh-Begriff(e) Animals ; Biological Evolution ; Mass Spectrometry ; Pattern Recognition, Automated ; Proteome/metabolism ; Proteomics/methods ; Snake Venoms/analysis
    Chemische Substanzen Proteome ; Snake Venoms
    Sprache Englisch
    Erscheinungsdatum 2018-07-20
    Erscheinungsland England
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2299100-1
    ISSN 1744-8387 ; 1478-9450
    ISSN (online) 1744-8387
    ISSN 1478-9450
    DOI 10.1080/14789450.2018.1500904
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  5. Artikel: The Secondary Myelodysplastic Neoplasms (MDS) Jigsaw.

    Calvete, Oriol / Mestre, Julia / Jerez, Andrés / Solé, Francesc

    Cancers

    2023  Band 15, Heft 5

    Abstract: There is a great deal of controversy in the hematologic community regarding the classification of secondary myelodysplastic neoplasms (MDSs). Current classifications are based on the presence of genetic predisposition and MDS post-cytotoxic therapy (MDS- ... ...

    Abstract There is a great deal of controversy in the hematologic community regarding the classification of secondary myelodysplastic neoplasms (MDSs). Current classifications are based on the presence of genetic predisposition and MDS post-cytotoxic therapy (MDS-pCT) etiologies. However, since these risk factors are not exclusive for secondary MDSs and there are multiple overlapping scenarios, a comprehensive and definitive classification is yet to come. In addition, a sporadic MDS might arise after a primary tumor fulfills the diagnostic criteria of MDS-pCT without a causative cytotoxicity. In this review, we describe the triggering pieces of a secondary MDS jigsaw: previous cytotoxic therapy, germline predisposition and clonal hematopoiesis. Epidemiological and translational efforts are needed to put these pieces together and ascertain the real weight of each of these pieces in each MDS patient. Future classifications must contribute to understanding the role of secondary MDS jigsaw pieces in different concomitant or independent clinical scenarios associated with the primary tumor.
    Sprache Englisch
    Erscheinungsdatum 2023-02-26
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article ; Review
    ZDB-ID 2527080-1
    ISSN 2072-6694
    ISSN 2072-6694
    DOI 10.3390/cancers15051483
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  6. Artikel ; Online: Longitudinal Associations Between Nonsuicidal Self-Injury, Depressive Symptoms, Hopelessness, and Emotional Dysregulation in Adolescents.

    Faura-Garcia, Juan / Calvete, Esther / Orue, Izaskun

    Archives of suicide research : official journal of the International Academy for Suicide Research

    2023  , Seite(n) 1–15

    Abstract: Nonsuicidal self-injury (NSSI) in adolescents has been associated with increased depressive symptomatology, hopelessness, and emotional dysregulation; however, few studies have examined longitudinal associations between NSSI and these problems. This ... ...

    Abstract Nonsuicidal self-injury (NSSI) in adolescents has been associated with increased depressive symptomatology, hopelessness, and emotional dysregulation; however, few studies have examined longitudinal associations between NSSI and these problems. This study examines the longitudinal relationships among these variables in community adolescents and whether the pattern of relationships varies between boys and girls. The participants were 785 adolescents (57.1% girls) aged 13 to 18 years (
    Sprache Englisch
    Erscheinungsdatum 2023-07-27
    Erscheinungsland England
    Dokumenttyp Journal Article
    ZDB-ID 1283671-0
    ISSN 1543-6136 ; 1381-1118
    ISSN (online) 1543-6136
    ISSN 1381-1118
    DOI 10.1080/13811118.2023.2237075
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  7. Artikel ; Online: Venomics: integrative venom proteomics and beyond.

    Calvete, Juan J

    The Biochemical journal

    2017  Band 474, Heft 5, Seite(n) 611–634

    Abstract: Venoms are integrated phenotypes that evolved independently in, and are used for predatory and defensive purposes by, a wide phylogenetic range of organisms. The same principles that contribute to the evolutionary success of venoms, contribute to making ... ...

    Abstract Venoms are integrated phenotypes that evolved independently in, and are used for predatory and defensive purposes by, a wide phylogenetic range of organisms. The same principles that contribute to the evolutionary success of venoms, contribute to making the study of venoms of great interest in such diverse fields as evolutionary ecology and biotechnology. Evolution is profoundly contingent, and nature also reinvents itself continuosly. Changes in a complex phenotypic trait, such as venom, reflect the influences of prior evolutionary history, chance events, and selection. Reconstructing the natural history of venoms, particularly those of snakes, which will be dealt with in more detail in this review, requires the integration of different levels of knowledge into a meaningful and comprehensive evolutionary framework for separating stochastic changes from adaptive evolution. The application of omics technologies and other disciplines have contributed to a qualitative and quantitative advance in the road map towards this goal. In this review we will make a foray into the world of animal venoms, discuss synergies and complementarities of the different approaches used in their study, and identify current bottlenecks that prevent inferring the evolutionary mechanisms and ecological constraints that molded snake venoms to their present-day variability landscape.
    Mesh-Begriff(e) Adaptation, Physiological ; Animals ; Antivenins/biosynthesis ; Biological Evolution ; Phospholipases A2/chemistry ; Phospholipases A2/isolation & purification ; Phospholipases A2/toxicity ; Phylogeny ; Protein Conformation ; Proteome ; Proteomics/methods ; Snake Venoms/chemistry ; Snakes/classification ; Snakes/physiology ; Transcriptome
    Chemische Substanzen Antivenins ; Proteome ; Snake Venoms ; Phospholipases A2 (EC 3.1.1.4)
    Sprache Englisch
    Erscheinungsdatum 2017-02-20
    Erscheinungsland England
    Dokumenttyp Journal Article ; Review
    ZDB-ID 2969-5
    ISSN 1470-8728 ; 0006-2936 ; 0306-3275 ; 0264-6021
    ISSN (online) 1470-8728
    ISSN 0006-2936 ; 0306-3275 ; 0264-6021
    DOI 10.1042/BCJ20160577
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  8. Artikel ; Online: Quantification of snake venom proteomes by mass spectrometry-considerations and perspectives.

    Calvete, Juan J / Lomonte, Bruno / Saviola, Anthony J / Calderón Celis, Francisco / Ruiz Encinar, Jorge

    Mass spectrometry reviews

    2023  

    Abstract: The advent of soft ionization mass spectrometry-based proteomics in the 1990s led to the development of a new dimension in biology that conceptually allows for the integral analysis of whole proteomes. This transition from a reductionist to a global- ... ...

    Abstract The advent of soft ionization mass spectrometry-based proteomics in the 1990s led to the development of a new dimension in biology that conceptually allows for the integral analysis of whole proteomes. This transition from a reductionist to a global-integrative approach is conditioned to the capability of proteomic platforms to generate and analyze complete qualitative and quantitative proteomics data. Paradoxically, the underlying analytical technique, molecular mass spectrometry, is inherently nonquantitative. The turn of the century witnessed the development of analytical strategies to endow proteomics with the ability to quantify proteomes of model organisms in the sense of "an organism for which comprehensive molecular (genomic and/or transcriptomic) resources are available." This essay presents an overview of the strategies and the lights and shadows of the most popular quantification methods highlighting the common misuse of label-free approaches developed for model species' when applied to quantify the individual components of proteomes of nonmodel species (In this essay we use the term "non-model" organisms for species lacking comprehensive molecular (genomic and/or transcriptomic) resources, a circumstance that, as we detail in this review-essay, conditions the quantification of their proteomes.). We also point out the opportunity of combining elemental and molecular mass spectrometry systems into a hybrid instrumental configuration for the parallel identification and absolute quantification of venom proteomes. The successful application of this novel mass spectrometry configuration in snake venomics represents a proof-of-concept for a broader and more routine application of hybrid elemental/molecular mass spectrometry setups in other areas of the proteomics field, such as phosphoproteomics, metallomics, and in general in any biological process where a heteroatom (i.e., any atom other than C, H, O, N) forms integral part of its mechanism.
    Sprache Englisch
    Erscheinungsdatum 2023-05-08
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Review
    ZDB-ID 1491946-1
    ISSN 1098-2787 ; 0277-7037
    ISSN (online) 1098-2787
    ISSN 0277-7037
    DOI 10.1002/mas.21850
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  9. Artikel ; Online: Proteomic analysis of the mandibular glands from the Chinese crocodile lizard, Shinisaurus crocodilurus – Another venomous lizard?

    Calvete, Juan J. / Lomonte, Bruno / Lorente, Carolina / Pla, Davinia / Zollweg, Michael / Mebs, D.

    Toxicon. 2023 Mar., v. 225 p.107050-

    2023  

    Abstract: Based on its phylogenetic relationship to monitor lizards (Varanidae), Gila monsters (Heloderma spp.), and the earless monitor Lanthanotus borneesis, the Chinese crocodile lizard, Shinisaurus crocodilurus, has been assigned to the Toxicofera clade, which ...

    Abstract Based on its phylogenetic relationship to monitor lizards (Varanidae), Gila monsters (Heloderma spp.), and the earless monitor Lanthanotus borneesis, the Chinese crocodile lizard, Shinisaurus crocodilurus, has been assigned to the Toxicofera clade, which comprises venomous reptiles. However, no data about composition and biological activities of its oral secretion have been reported. In the present study, a proteomic analysis of the mandibular gland of S. crocodilurus and, for comparison, of the herbivorous Solomon Island skink Corucia zebrata, was performed. Scanning electron microscopy (SEM) of the teeth from S. crocodilurus revealed a sharp ridge on the anterior surface, but no grooves, whereas those of C. zebrata possess a flattened crown with a pointed cusp. Proteomic analysis of their gland extracts provided no evidence of venom-derived peptides or proteins, strongly supporting the non-venomous character of these lizards. Data are available via ProteomeXchange with identifier PXD039424.
    Schlagwörter Corucia zebrata ; Gila ; Heloderma ; Varanidae ; crocodiles ; electron microscopy ; herbivores ; lizards ; peptides ; phylogeny ; proteomics ; secretion ; Chinese crocodile lizard ; Shinisaurus crocodilurus ; Solomon Island skink ; Mandibular gland ; Proteomic analysis
    Sprache Englisch
    Erscheinungsverlauf 2023-03
    Erscheinungsort Elsevier Ltd
    Dokumenttyp Artikel ; Online
    Anmerkung Use and reproduction
    ZDB-ID 204479-1
    ISSN 1879-3150 ; 0041-0101
    ISSN (online) 1879-3150
    ISSN 0041-0101
    DOI 10.1016/j.toxicon.2023.107050
    Datenquelle NAL Katalog (AGRICOLA)

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  10. Artikel ; Online: The challenge of integrating proximate and ultimate causes to reconstruct the natural histories of venoms: the evolutionary link.

    Calvete, Juan J

    Expert review of proteomics

    2016  Band 13, Heft 12, Seite(n) 1059–1061

    Sprache Englisch
    Erscheinungsdatum 2016-12
    Erscheinungsland England
    Dokumenttyp Journal Article
    ZDB-ID 2299100-1
    ISSN 1744-8387 ; 1478-9450
    ISSN (online) 1744-8387
    ISSN 1478-9450
    DOI 10.1080/14789450.2017.1242416
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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