Artikel ; Online: Assessing Interleukin-1β Activation During Pyroptosis.
Methods in molecular biology (Clifton, N.J.)
2023 Band 2641, Seite(n) 163–169
Abstract: The cytokine interleukin-1β (IL-1β) is a major mediator of inflammation in autoinflammatory disease and the host response to infection. IL-1β is stored within cells in an inert form, which requires proteolytic removal of an amino-terminal fragment in ... ...
Abstract | The cytokine interleukin-1β (IL-1β) is a major mediator of inflammation in autoinflammatory disease and the host response to infection. IL-1β is stored within cells in an inert form, which requires proteolytic removal of an amino-terminal fragment in order to bind the IL-1 receptor complex and have pro-inflammatory activity. This cleavage event is canonically carried out by inflammasome-activated caspase proteases, but microbe and host proteases can also generate unique active forms. Post-translational regulation of IL-1β and the diversity in resulting products can present challenges to the evaluation of IL-1β activation. This chapter details methods and important controls for the accurate and sensitive measurement of IL-1β activation within biological samples. |
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Mesh-Begriff(e) | Pyroptosis/physiology ; Interleukin-1beta/metabolism ; Inflammasomes/metabolism ; Caspases ; NLR Family, Pyrin Domain-Containing 3 Protein/metabolism ; Caspase 1/metabolism |
Chemische Substanzen | Interleukin-1beta ; Inflammasomes ; Caspases (EC 3.4.22.-) ; NLR Family, Pyrin Domain-Containing 3 Protein ; Caspase 1 (EC 3.4.22.36) |
Sprache | Englisch |
Erscheinungsdatum | 2023-04-19 |
Erscheinungsland | United States |
Dokumenttyp | Journal Article ; Research Support, N.I.H., Extramural |
ISSN | 1940-6029 |
ISSN (online) | 1940-6029 |
DOI | 10.1007/978-1-0716-3040-2_13 |
Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
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