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  1. Buch: Microfluidics and biosensors in cancer research

    Caballero, David / Kundu, S. C. / Reis, Rui L.

    applications in cancer modeling and theranostics

    (Advances in experimental medicine and biology ; 1379)

    2022  

    Verfasserangabe David Caballero, Subhas C. Kundu, Rui L. Reis editors
    Serientitel Advances in experimental medicine and biology ; 1379
    Überordnung
    Schlagwörter Cancer/Research/Technological innovations ; Biosensors ; Microfluidic devices
    Thema/Rubrik (Code) 616.99400285
    Sprache Englisch
    Umfang xix, 590 Seiten, Illustrationen, 24 cm
    Verlag Springer
    Erscheinungsort Cham
    Erscheinungsland Schweiz
    Dokumenttyp Buch
    HBZ-ID HT021410198
    ISBN 978-3-031-04038-2 ; 9783031040399 ; 3-031-04038-4 ; 3031040392
    Datenquelle Katalog ZB MED Medizin, Gesundheit

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  2. Buch ; Online ; E-Book: Biomaterials for 3D tumor modeling

    Kundu, S. C. / Reis, Rui L.

    (Materials today)

    2020  

    Verfasserangabe edited by Subhas C. Kundu, Rui L. Reis
    Serientitel Materials today
    Schlagwörter Tumors ; Three-dimensional imaging in medicine ; Biomedical materials
    Thema/Rubrik (Code) 616.994027
    Sprache Englisch
    Umfang 1 Online-Ressource (753 Seiten), Illustrationen
    Verlag Elsevier
    Erscheinungsort Amsterdam
    Erscheinungsland Niederlande
    Dokumenttyp Buch ; Online ; E-Book
    Bemerkung Zugriff für angemeldete ZB MED-Nutzerinnen und -Nutzer
    HBZ-ID HT020670197
    ISBN 978-0-12-818129-4 ; 9780128181287 ; 0-12-818129-X ; 0128181281
    Datenquelle ZB MED Katalog Medizin, Gesundheit, Ernährung, Umwelt, Agrar

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  3. Artikel ; Online: Radical Prostatectomy Technique Dispute: Analyzing Over 1.35 Million Surgeries in 20 Years of History.

    Moretti, Tomás B C / Magna, Luís A / Reis, Leonardo O

    Clinical genitourinary cancer

    2023  Band 21, Heft 4, Seite(n) e271–e278.e42

    Abstract: Systematic reviews (SR) produce the best evidence comparing open (RRP), laparoscopic (LRP), and robotic (RARP) radical prostatectomy (RP). However, the hyperfiltration of evidence generates very specific scenarios that reduce the power of extrapolation. ... ...

    Abstract Systematic reviews (SR) produce the best evidence comparing open (RRP), laparoscopic (LRP), and robotic (RARP) radical prostatectomy (RP). However, the hyperfiltration of evidence generates very specific scenarios that reduce the power of extrapolation. To compare RP evidence regarding demographics using a new methodology called reverse systematic review (RSR). Between 2000 and 2020, 8 databases were searched for SR studies on RRP, LRP, or RARP. All references were captured and analyzed over time in 80 SR. Total of 1724 reports (n
    Mesh-Begriff(e) Male ; Humans ; Dissent and Disputes ; Treatment Outcome ; Prostate ; Robotic Surgical Procedures/methods ; Prostatectomy/methods ; Laparoscopy/methods
    Sprache Englisch
    Erscheinungsdatum 2023-02-16
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Review ; Research Support, Non-U.S. Gov't
    ZDB-ID 2225121-2
    ISSN 1938-0682 ; 1558-7673
    ISSN (online) 1938-0682
    ISSN 1558-7673
    DOI 10.1016/j.clgc.2023.02.005
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  4. Artikel ; Online: Continence criteria of 193 618 patients after open, laparoscopic, and robot-assisted radical prostatectomy.

    Moretti, Tomás B C / Magna, Luís A / Reis, Leonardo O

    BJU international

    2023  

    Abstract: Objectives: To apply a new evidence-gathering methodology, called reverse systematic review (RSR), to analyse the influence of different continence classification criteria on urinary continence rates among open retropubic radical prostatectomy (RRP), ... ...

    Abstract Objectives: To apply a new evidence-gathering methodology, called reverse systematic review (RSR), to analyse the influence of different continence classification criteria on urinary continence rates among open retropubic radical prostatectomy (RRP), laparoscopic RP (LRP) and robot-assisted RP (RARP).
    Materials and methods: A search was carried out in eight databases between 2000 and 2020 through systematic reviews (SRs) studies referring to RRP, LRP or RARP (80 SRs). All references used in these SRs were captured referring to 910 papers in an overall database called the 'EVIDENCE Database'. A total of 422 studies related to post-RP urinary continence were selected for the final analysis, totalling 782 reports referring to 193 618 patients.
    Results: Overall, 206 (26.4%) reports for RRP, 243 (31.0%) reports for LRP, and 333 (42.6%) reports for RARP were found. Mean overall continence rates, respectively for RRP, LRP and RARP, were: 42%, 34% and 42% at 1 month; 62%, 64% and 65% at 3 months; 73, 77 and 79% at 6 months; and 81%, 85% and 86% at 12 months. The most used criterion was 'No pad' (53.3%), followed by 'Safety pad' (19.3%), 'Not described' (10.6%), and 'No leak' (9.9%). 'No pad' showed the lowest discrepancy in continence rates in each period compared to the overall average for each technique, demonstrating less ability to influence the final results favouring any of the techniques.
    Conclusion: The RSR demonstrated that the 'No pad' criterion was the most used in the literature and showed the lowest bias capable of influencing the results and favouring any of the techniques and is the fairest option for future comparisons.
    Sprache Englisch
    Erscheinungsdatum 2023-09-15
    Erscheinungsland England
    Dokumenttyp Journal Article ; Review
    ZDB-ID 1462191-5
    ISSN 1464-410X ; 1464-4096 ; 1358-8672
    ISSN (online) 1464-410X
    ISSN 1464-4096 ; 1358-8672
    DOI 10.1111/bju.16180
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  5. Artikel: Numerical Simulations of the Low-Velocity Impact Response of Semicylindrical Woven Composite Shells.

    Ferreira, Luis M / Coelho, Carlos A C P / Reis, Paulo N B

    Materials (Basel, Switzerland)

    2023  Band 16, Heft 9

    Abstract: This paper presents an efficient and reliable approach to study the low-velocity impact response of woven composite shells using 3D finite element models that account for the physical intralaminar and interlaminar progressive damage. The authors' ... ...

    Abstract This paper presents an efficient and reliable approach to study the low-velocity impact response of woven composite shells using 3D finite element models that account for the physical intralaminar and interlaminar progressive damage. The authors' previous work on the experimental assessment of the effect of thickness on the impact response of semicylindrical composite laminated shells served as the basis for this paper. Therefore, the finite element models were put to the test in comparison to the experimental findings. A good agreement was obtained between the numerical predictions and experimental data for the load and energy histories as well as for the maximum impact load, maximum displacement, and contact time. The use of the mass-scaling technique was successfully implemented, reducing considerably the computing cost of the solutions. The maximum load, maximum displacement, and contact time are negligibly affected by the choice of finite element mesh discretization. However, it has an impact on the initiation and progression of interlaminar damage. Therefore, to accurately compute delamination, its correct definition is of upmost importance. The validation of these finite element models opens the possibility for further numerical studies on of woven composite shells and enables shortening the time and expenses associated with the experimental testing.
    Sprache Englisch
    Erscheinungsdatum 2023-04-28
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2487261-1
    ISSN 1996-1944
    ISSN 1996-1944
    DOI 10.3390/ma16093442
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  6. Artikel ; Online: Multicomponent oxide microspheres with designed macroporosity (MICROSCAFS®): a customized platform for chemicals immobilization.

    Vale, Mário / Orišková, Sofia / Mariquito, António / Reis, Luís / Pinto, Moisés / Marques, Ana C

    RSC advances

    2023  Band 13, Heft 19, Seite(n) 12951–12965

    Abstract: A novel versatile, easily recoverable, and recyclable material platform is herein presented, consisting of multicomponent oxide microspheres, of silica-titania and silica-titania-hafnia composition, with tailored interconnected macroporosity (MICROSCAFS®) ...

    Abstract A novel versatile, easily recoverable, and recyclable material platform is herein presented, consisting of multicomponent oxide microspheres, of silica-titania and silica-titania-hafnia composition, with tailored interconnected macroporosity (MICROSCAFS®). When functionalized or loaded with desired species, they are potential enablers of emerging applications in environmental remediation, among other fields. We combine emulsion templating for the spherical shape of the particles, with an adapted sol-gel technique involving polymerization-induced phase separation by spinodal decomposition. An advantage of our method regards the employed mix of precursors, which prevents the use of specific gelation additives and porogens and allows a high reproducibility of MICROSCAFS®. We present insight into their formation mechanism using cryo-scanning electron microscopy, and a systematic study of the effect of multiple synthesis parameters on the MICROSCAFS® size and porosity. The composition of the silicon precursors has the most significant effect on fine-tuning the pores size, ranging from the nanometer to the micron scale. Mechanical properties are correlated with morphological features. Larger macroporosity (68% open porosity, estimated by X-ray computed tomography) leads to less stiffness, higher elastic recovery, and compressibility values up to 42%. We believe this study creates a base for consistent custom MICROSCAFS® production, with a design for various future applications.
    Sprache Englisch
    Erscheinungsdatum 2023-04-25
    Erscheinungsland England
    Dokumenttyp Journal Article
    ISSN 2046-2069
    ISSN (online) 2046-2069
    DOI 10.1039/d3ra00895a
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  7. Artikel ; Online: Effect of Cohesive Properties on Low-Velocity Impact Simulations of Woven Composite Shells

    Luis M. Ferreira / Carlos A. C. P. Coelho / Paulo N. B. Reis

    Applied Sciences, Vol 13, Iss 6948, p

    2023  Band 6948

    Abstract: The effect of the interface stiffness and interface strength on the low-velocity impact response of woven-fabric semicylindrical composite shells is studied using finite element (FE) models generated with continuum shell elements and cohesive surfaces. ... ...

    Abstract The effect of the interface stiffness and interface strength on the low-velocity impact response of woven-fabric semicylindrical composite shells is studied using finite element (FE) models generated with continuum shell elements and cohesive surfaces. The intralaminar damage is accounted for using the constitutive model provided within the ABAQUS software, while the interlaminar is addressed utilising cohesive surfaces. The results show that the interface stiffness has a negligible effect on the force and energy histories for values between 10 <semantics> 1 </semantics> N/mm <semantics> 3 </semantics> and 2.43 × 10 <semantics> 6 </semantics> N/mm <semantics> 3 </semantics> . However, it has a significant impact on the delamination predictions. It is observed that only the normal interface strength affects the maximum impact force and the delamination predictions. Increasing its value from 15 MPa to 30 MPa resulted in an 8% growth in the maximum force, and a substantial reduction in the delaminated area. The obtained results serve as guidelines for the accurate and efficient computation of delamination. The successful validation of the FE models establishes a solid foundation for further numerical investigations and offers the potential to significantly reduce the time and expenses associated with experimental testing.
    Schlagwörter low-velocity impact ; cohesive behaviour ; interlaminar properties ; finite element method ; composite shells ; Technology ; T ; Engineering (General). Civil engineering (General) ; TA1-2040 ; Biology (General) ; QH301-705.5 ; Physics ; QC1-999 ; Chemistry ; QD1-999
    Thema/Rubrik (Code) 511
    Sprache Englisch
    Erscheinungsdatum 2023-06-01T00:00:00Z
    Verlag MDPI AG
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  8. Artikel ; Online: Prediction of enzymatic function with high efficiency and a reduced number of features using genetic algorithm.

    Reis, Diogo R / Santos, Bruno C / Bleicher, Lucas / Zárate, Luis E / Nobre, Cristiane N

    Computers in biology and medicine

    2023  Band 158, Seite(n) 106799

    Abstract: The post-genomic era has raised a growing demand for efficient procedures to identify protein functions, which can be accomplished by applying machine learning to the characteristics set extracted from the protein. This approach is feature-based and has ... ...

    Abstract The post-genomic era has raised a growing demand for efficient procedures to identify protein functions, which can be accomplished by applying machine learning to the characteristics set extracted from the protein. This approach is feature-based and has been the focus of several works in bioinformatics. In this work, we investigated the characteristics of proteins, representing the primary, secondary, tertiary, and quaternary structures of the protein, that improve the model's quality by applying dimensionality reduction techniques and using the Support Vector Machine classifier for predicting the enzymes' classes. During the investigation, two approaches were evaluated: feature extraction/transformation, which was performed using the statistical technique Factor Analysis, and feature selection methods. For feature selection, we proposed an approach based on a genetic algorithm to face the optimization conflict between the simplicity and reliability of an ideal representation of the characteristics of the enzymes and also compared and employed other methods for this purpose. The best result was accomplished using a feature subset generated by our implementation of a multi-objective genetic algorithm enriched with features that this work identified as relevant to represent the enzymes. This subset representation reduced the dataset by about 87% and reached 85.78% of F-measure performance, improving the overall quality of the model classification. In addition, we verified in this work a subset addressed with only 28 features out of a total of 424 that reached a performance above 80% of F-measure for four of the six evaluated classes, showing that satisfactory classification performance can be achieved with a reduced number of enzymes's characteristics. The datasets and implementations are openly available.
    Mesh-Begriff(e) Reproducibility of Results ; Machine Learning ; Proteins ; Computational Biology ; Genomics ; Support Vector Machine ; Algorithms
    Chemische Substanzen Proteins
    Sprache Englisch
    Erscheinungsdatum 2023-03-22
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 127557-4
    ISSN 1879-0534 ; 0010-4825
    ISSN (online) 1879-0534
    ISSN 0010-4825
    DOI 10.1016/j.compbiomed.2023.106799
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  9. Artikel: Water deprivation induces hypoactivity in rats independently of oxytocin receptor signaling at the central amygdala.

    Felintro, Viviane / Trujillo, Verónica / Dos-Santos, Raoni C / da Silva-Almeida, Claudio / Reis, Luís C / Rocha, Fábio F / Mecawi, André S

    Frontiers in endocrinology

    2023  Band 14, Seite(n) 1062211

    Abstract: Introduction: Vasopressin (AVP) and oxytocin (OXT) are neuropeptides produced by magnocellular neurons (MCNs) of the hypothalamus and secreted through neurohypophysis to defend mammals against dehydration. It was recently demonstrated that MCNs also ... ...

    Abstract Introduction: Vasopressin (AVP) and oxytocin (OXT) are neuropeptides produced by magnocellular neurons (MCNs) of the hypothalamus and secreted through neurohypophysis to defend mammals against dehydration. It was recently demonstrated that MCNs also project to limbic structures, modulating several behavioral responses.
    Methods and results: We found that 24 h of water deprivation (WD) or salt loading (SL) did not change exploration or anxiety-like behaviors in the elevated plus maze (EPM) test. However, rats deprived of water for 48 h showed reduced exploration of open field and the closed arms of EPM, indicating hypoactivity during night time. We evaluated mRNA expression of glutamate decarboxylase 1 (Gad1), vesicular glutamate transporter 2 (Slc17a6), AVP (Avpr1a) and OXT (Oxtr) receptors in the lateral habenula (LHb), basolateral (BLA) and central (CeA) amygdala after 48 h of WD or SL. WD, but not SL, increased Oxtr mRNA expression in the CeA. Bilateral pharmacological inhibition of OXTR function in the CeA with the OXTR antagonist L-371,257 was performed to evaluate its possible role in regulating the EPM exploration or water intake induced by WD. The blockade of OXTR in the CeA did not reverse the hypoactivity response in the EPM, nor did it change water intake induced in 48-h water-deprived rats.
    Discussion: We found that WD modulates exploratory activity in rats, but this response is not mediated by oxytocin receptor signaling to the CeA, despite the upregulated Oxtr mRNA expression in that structure after WD for 48 h.
    Mesh-Begriff(e) Rats ; Animals ; Central Amygdaloid Nucleus/metabolism ; Oxytocin/metabolism ; Receptors, Oxytocin/metabolism ; Dehydration ; Water Deprivation ; Water ; RNA, Messenger ; Mammals/metabolism
    Chemische Substanzen Oxytocin (50-56-6) ; Receptors, Oxytocin ; Water (059QF0KO0R) ; RNA, Messenger
    Sprache Englisch
    Erscheinungsdatum 2023-01-31
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2592084-4
    ISSN 1664-2392
    ISSN 1664-2392
    DOI 10.3389/fendo.2023.1062211
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  10. Artikel ; Online: Dietary glycemic and energy load differentially modulates Schistosoma mansoni-induced granulomatous inflammation and response to antiparasitic chemotherapy.

    Reis, Luis F C Dos / Cerdeira, Cláudio D / Silva, Laís C C / Ramos, Amanda B S B / Silva, José Edson C / Castro, Aline P / Ventura, Renato R / Souza, Raquel L M / Marques, Marcos J / Novaes, Rômulo D

    Acta tropica

    2024  Band 252, Seite(n) 107141

    Abstract: ... c mice were infected with S. mansoni and followed for 15 weeks. The animals were randomized ...

    Abstract The impact of diet composition and energy content on schistosomiasis evolution and treatment efficacy is still controversial. This study compared the impact of sucrose-rich diet and intermittent fasting on Schistosoma mansoni infection and praziquantel (PZQ)-based chemotherapy response in mice. BALB/c mice were infected with S. mansoni and followed for 15 weeks. The animals were randomized into nine groups receiving high glycemic load (high-sucrose diet - HSD), low caloric load (standard chow alternate-day fasting - ADF), and standard chow ad libitum (AL). Eight weeks after S. mansoni infection, these groups remained untreated or were treated with PZQ (300 mg/kg/day) for 3 days. Our results indicated that parasite load (S. mansoni eggs and parasite DNA levels), granulomatous inflammation (granulomas number and size), and liver microstructural damage (reduction in hepatocytes number, increase in nucleus-cytoplasm ratio, connective stroma expansion and fibrosis) were increased in ADF-treated animals. These animals also showed decreased eggs retention, granulomatous inflammation and collagen accumulation in the small intestine. Conversely, HSD diet and PZQ treatment attenuated all these parameters and stimulated hepatic regenerative response. PZQ also stimulated fibrosis resolution in HSD-treated mice, effect that was limited ADF-exposed mice. Our findings indicate that dietary glycemic and energy load can modulate schistosomiasis progression and the severity of hepatic and intestinal granulomatous inflammation in untreated and PZQ-treated mice. Thus, lower intestinal eggs retention may potentially be linked to worsening liver disease in ADF, while attenuation of hepatic and intestinal granulomatous inflammation is consistent with reduced parasite load in HSD- and PZQ-treated animals.
    Mesh-Begriff(e) Animals ; Mice ; Schistosoma mansoni ; Antiparasitic Agents/therapeutic use ; Praziquantel/pharmacology ; Schistosomiasis mansoni/drug therapy ; Schistosomiasis mansoni/parasitology ; Liver/parasitology ; Liver Diseases ; Schistosomiasis/drug therapy ; Inflammation/drug therapy ; Fibrosis ; Diet ; Sucrose/pharmacology ; Sucrose/therapeutic use ; Anthelmintics/therapeutic use
    Chemische Substanzen Antiparasitic Agents ; Praziquantel (6490C9U457) ; Sucrose (57-50-1) ; Anthelmintics
    Sprache Englisch
    Erscheinungsdatum 2024-02-10
    Erscheinungsland Netherlands
    Dokumenttyp Journal Article
    ZDB-ID 210415-5
    ISSN 1873-6254 ; 0001-706X
    ISSN (online) 1873-6254
    ISSN 0001-706X
    DOI 10.1016/j.actatropica.2024.107141
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