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Artikel ; Online: Live imaging of the

Wilcockson, Scott G / Ashe, Hilary L

STAR protocols

2021 Band 2, Heft 1, Seite(n) 100371

Abstract: The maintenance of stem cell populations and the differentiation of their progeny is coordinated by specific communication with associated niche cells. Here, we describe a protocol for short-term live imaging of ... ...

Abstract	The maintenance of stem cell populations and the differentiation of their progeny is coordinated by specific communication with associated niche cells. Here, we describe a protocol for short-term live imaging of the
Mesh-Begriff(e)	Adult Germline Stem Cells/cytology ; Animals ; Cell Differentiation ; Drosophila/cytology ; Female ; Germ Cells/cytology ; Molecular Imaging/methods ; Oogonial Stem Cells/cytology ; Ovary/cytology ; Stem Cell Niche/physiology ; Stem Cells/cytology
Sprache	Englisch
Erscheinungsdatum	2021-03-05
Erscheinungsland	United States
Dokumenttyp	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	2666-1667
ISSN (online)	2666-1667
DOI	10.1016/j.xpro.2021.100371
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Context-dependent TGFβ family signalling in cell fate regulation.

Richardson, Louise / Wilcockson, Scott G / Guglielmi, Luca / Hill, Caroline S

Nature reviews. Molecular cell biology

2023 Band 24, Heft 12, Seite(n) 876–894

Abstract: The transforming growth factor-β (TGFβ) family are a large group of evolutionarily conserved cytokines whose signalling modulates cell fate decision-making across varying cellular contexts at different stages of life. Here we discuss new findings in ... ...

Abstract	The transforming growth factor-β (TGFβ) family are a large group of evolutionarily conserved cytokines whose signalling modulates cell fate decision-making across varying cellular contexts at different stages of life. Here we discuss new findings in early embryos that reveal how, in contrast to our original understanding of morphogen interpretation, robust cell fate specification can originate from a noisy combination of signalling inputs and a broad range of signalling levels. We compare this evidence with novel findings on the roles of TGFβ family signalling in tissue maintenance and homeostasis during juvenile and adult life, spanning the skeletal, haemopoietic and immune systems. From these comparisons, it emerges that in contrast to robust developing systems, relatively small perturbations in TGFβ family signalling have detrimental effects at later stages in life, leading to aberrant cell fate specification and disease, for example in cancer or congenital disorders. Finally, we highlight novel strategies to target and amend dysfunction in signalling and discuss how gleaning knowledge from different fields of biology can help in the development of therapeutics for aberrant TGFβ family signalling in disease.
Mesh-Begriff(e)	Humans ; Transforming Growth Factor beta ; Signal Transduction/physiology ; Neoplasms
Chemische Substanzen	Transforming Growth Factor beta
Sprache	Englisch
Erscheinungsdatum	2023-08-18
Erscheinungsland	England
Dokumenttyp	Journal Article ; Review
ZDB-ID	2031313-5
ISSN	1471-0080 ; 1471-0072
ISSN (online)	1471-0080
ISSN	1471-0072
DOI	10.1038/s41580-023-00638-3
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: An improved Erk biosensor detects oscillatory Erk dynamics driven by mitotic erasure during early development.

Wilcockson, Scott G / Guglielmi, Luca / Araguas Rodriguez, Pablo / Amoyel, Marc / Hill, Caroline S

Developmental cell

2023 Band 58, Heft 23, Seite(n) 2802–2818.e5

Abstract: Extracellular signal-regulated kinase (Erk) signaling dynamics elicit distinct cellular responses in a variety of contexts. The early zebrafish embryo is an ideal model to explore the role of Erk signaling dynamics in vivo, as a gradient of activated ... ...

Abstract	Extracellular signal-regulated kinase (Erk) signaling dynamics elicit distinct cellular responses in a variety of contexts. The early zebrafish embryo is an ideal model to explore the role of Erk signaling dynamics in vivo, as a gradient of activated diphosphorylated Erk (P-Erk) is induced by fibroblast growth factor (Fgf) signaling at the blastula margin. Here, we describe an improved Erk-specific biosensor, which we term modified Erk kinase translocation reporter (modErk-KTR). We demonstrate the utility of this biosensor in vitro and in developing zebrafish and Drosophila embryos. Moreover, we show that Fgf/Erk signaling is dynamic and coupled to tissue growth during both early zebrafish and Drosophila development. Erk activity is rapidly extinguished just prior to mitosis, which we refer to as mitotic erasure, inducing periods of inactivity, thus providing a source of heterogeneity in an asynchronously dividing tissue. Our modified reporter and transgenic lines represent an important resource for interrogating the role of Erk signaling dynamics in vivo.
Mesh-Begriff(e)	Animals ; Extracellular Signal-Regulated MAP Kinases/metabolism ; Zebrafish/metabolism ; Signal Transduction ; Fibroblast Growth Factors/metabolism ; Drosophila/metabolism ; Biosensing Techniques
Chemische Substanzen	Extracellular Signal-Regulated MAP Kinases (EC 2.7.11.24) ; Fibroblast Growth Factors (62031-54-3)
Sprache	Englisch
Erscheinungsdatum	2023-09-14
Erscheinungsland	United States
Dokumenttyp	Journal Article
ZDB-ID	2054967-2
ISSN	1878-1551 ; 1534-5807
ISSN (online)	1878-1551
ISSN	1534-5807
DOI	10.1016/j.devcel.2023.08.021
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Drosophila Ovarian Germline Stem Cell Cytocensor Projections Dynamically Receive and Attenuate BMP Signaling.

Wilcockson, Scott G / Ashe, Hilary L

Developmental cell

2019 Band 50, Heft 3, Seite(n) 296–312.e5

Abstract: In the Drosophila ovarian germline, Bone Morphogenetic Protein (BMP) signals released by niche cells promote germline stem cell (GSC) maintenance. Although BMP signaling is known to repress expression of a key differentiation factor, it remains unclear ... ...

Abstract	In the Drosophila ovarian germline, Bone Morphogenetic Protein (BMP) signals released by niche cells promote germline stem cell (GSC) maintenance. Although BMP signaling is known to repress expression of a key differentiation factor, it remains unclear whether BMP-responsive transcription also contributes positively to GSC identity. Here, we identify the GSC transcriptome using RNA sequencing (RNA-seq), including the BMP-induced transcriptional network. Based on these data, we provide evidence that GSCs form two types of cellular projections. Genetic manipulation and live ex vivo imaging reveal that both classes of projection allow GSCs to access a reservoir of Dpp held away from the GSC-niche interface. Moreover, microtubule-rich projections, termed "cytocensors", form downstream of BMP and have additional functionality, which is to attenuate BMP signaling. In this way, cytocensors allow dynamic modulation of signal transduction to facilitate differentiation following GSC division. This ability of cytocensors to attenuate the signaling response expands the repertoire of functions associated with signaling projections.
Mesh-Begriff(e)	Animals ; Bone Morphogenetic Proteins/genetics ; Bone Morphogenetic Proteins/metabolism ; Cell Differentiation ; Drosophila Proteins/genetics ; Drosophila Proteins/metabolism ; Drosophila melanogaster ; Female ; Gene Expression Regulation, Developmental ; Ovary/cytology ; Signal Transduction ; Stem Cell Niche ; Stem Cells/cytology ; Stem Cells/metabolism
Chemische Substanzen	Bone Morphogenetic Proteins ; Drosophila Proteins ; dpp protein, Drosophila
Sprache	Englisch
Erscheinungsdatum	2019-06-06
Erscheinungsland	United States
Dokumenttyp	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2054967-2
ISSN	1878-1551 ; 1534-5807
ISSN (online)	1878-1551
ISSN	1534-5807
DOI	10.1016/j.devcel.2019.05.020
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Live imaging of the Drosophila ovarian germline stem cell niche

Scott G. Wilcockson / Hilary L. Ashe

STAR Protocols, Vol 2, Iss 1, Pp 100371- (2021)

2021

Abstract: ... of this protocol, please refer to Wilcockson and Ashe (2019). ...

Abstract	Summary: The maintenance of stem cell populations and the differentiation of their progeny is coordinated by specific communication with associated niche cells. Here, we describe a protocol for short-term live imaging of the Drosophila ovarian germline stem cell niche ex vivo. By immobilizing the ovarian tissue in a fibrinogen-thrombin clot, we are able to maintain the tissue for short-term high-temporal live imaging. This enables the visualization of dynamic cellular processes, such as the cytoskeletal dynamics that control stem cell niche communication.For complete details on the use and execution of this protocol, please refer to Wilcockson and Ashe (2019).
Schlagwörter	Cell Biology ; Microscopy ; Model Organisms ; Stem Cells ; Science (General) ; Q1-390
Sprache	Englisch
Erscheinungsdatum	2021-03-01T00:00:00Z
Verlag	Elsevier
Dokumenttyp	Artikel ; Online
Datenquelle	BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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Artikel: Control of signaling molecule range during developmental patterning

Wilcockson, Scott G / Catherine Sutcliffe / Hilary L. Ashe

Cellular and molecular life sciences. 2017 June, v. 74, no. 11

2017

Abstract: Tissue patterning, through the concerted activity of a small number of signaling pathways, is critical to embryonic development. While patterning can involve signaling between neighbouring cells, in other contexts signals act over greater distances by ... ...

Abstract	Tissue patterning, through the concerted activity of a small number of signaling pathways, is critical to embryonic development. While patterning can involve signaling between neighbouring cells, in other contexts signals act over greater distances by traversing complex cellular landscapes to instruct the fate of distant cells. In this review, we explore different strategies adopted by cells to modulate signaling molecule range to allow correct patterning. We describe mechanisms for restricting signaling range and highlight how such short-range signaling can be exploited to not only control the fate of adjacent cells, but also to generate graded signaling within a field of cells. Other strategies include modulation of signaling molecule action by tissue architectural properties and the use of cellular membranous structures, such as signaling filopodia and exosomes, to actively deliver signaling ligands to target cells. Signaling filopodia can also be deployed to reach out and collect particular signals, thereby precisely controlling their site of action.
Schlagwörter	embryogenesis ; exosomes ; landscapes ; ligands ; pseudopodia ; signal transduction
Sprache	Englisch
Erscheinungsverlauf	2017-06
Umfang	p. 1937-1956.
Erscheinungsort	Springer International Publishing
Dokumenttyp	Artikel
Anmerkung	Review
ZDB-ID	1358415-7
ISSN	1420-9071 ; 1420-682X
ISSN (online)	1420-9071
ISSN	1420-682X
DOI	10.1007/s00018-016-2433-5
Datenquelle	NAL Katalog (AGRICOLA)

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Artikel ; Online: Control of signaling molecule range during developmental patterning.

Wilcockson, Scott G / Sutcliffe, Catherine / Ashe, Hilary L

Cellular and molecular life sciences : CMLS

2016 Band 74, Heft 11, Seite(n) 1937–1956

Abstract	Tissue patterning, through the concerted activity of a small number of signaling pathways, is critical to embryonic development. While patterning can involve signaling between neighbouring cells, in other contexts signals act over greater distances by traversing complex cellular landscapes to instruct the fate of distant cells. In this review, we explore different strategies adopted by cells to modulate signaling molecule range to allow correct patterning. We describe mechanisms for restricting signaling range and highlight how such short-range signaling can be exploited to not only control the fate of adjacent cells, but also to generate graded signaling within a field of cells. Other strategies include modulation of signaling molecule action by tissue architectural properties and the use of cellular membranous structures, such as signaling filopodia and exosomes, to actively deliver signaling ligands to target cells. Signaling filopodia can also be deployed to reach out and collect particular signals, thereby precisely controlling their site of action.
Mesh-Begriff(e)	Animals ; Body Patterning ; Cell Surface Extensions/metabolism ; Embryonic Development ; Extracellular Vesicles/metabolism ; Humans ; Ligands ; Signal Transduction
Chemische Substanzen	Ligands
Sprache	Englisch
Erscheinungsdatum	2016-12-20
Erscheinungsland	Switzerland
Dokumenttyp	Journal Article ; Review
ZDB-ID	1358415-7
ISSN	1420-9071 ; 1420-682X
ISSN (online)	1420-9071
ISSN	1420-682X
DOI	10.1007/s00018-016-2433-5
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Peak BMP Responses in the Drosophila Embryo Are Dependent on the Activation of Integrin Signaling.

Sawala, Annick / Scarcia, Margherita / Sutcliffe, Catherine / Wilcockson, Scott G / Ashe, Hilary L

Cell reports

2015 Band 13, Heft 7, Seite(n) 1519–1520

Sprache	Englisch
Erscheinungsdatum	2015-11-17
Erscheinungsland	United States
Dokumenttyp	Published Erratum
ZDB-ID	2649101-1
ISSN	2211-1247 ; 2211-1247
ISSN (online)	2211-1247
ISSN	2211-1247
DOI	10.1016/j.celrep.2015.10.079
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Peak BMP Responses in the Drosophila Embryo Are Dependent on the Activation of Integrin Signaling.

Sawala, Annick / Scarcia, Margherita / Sutcliffe, Catherine / Wilcockson, Scott G / Ashe, Hilary L

Cell reports

2015 Band 12, Heft 10, Seite(n) 1584–1593

Abstract: Within a 3D tissue, cells need to integrate signals from growth factors, such as BMPs, and the extracellular matrix (ECM) to coordinate growth and differentiation. Here, we use the Drosophila embryo as a model to investigate how BMP responses are ... ...

Abstract	Within a 3D tissue, cells need to integrate signals from growth factors, such as BMPs, and the extracellular matrix (ECM) to coordinate growth and differentiation. Here, we use the Drosophila embryo as a model to investigate how BMP responses are influenced by a cell's local ECM environment. We show that integrins, which are ECM receptors, are absolutely required for peak BMP signaling. This stimulatory effect of integrins requires their intracellular signaling function, which is activated by the ECM protein collagen IV. Mechanistically, integrins interact with the BMP receptor and stimulate phosphorylation of the downstream Mad transcription factor. The BMP-pathway-enhancing function of integrins is independent of focal adhesion kinase, but it requires conserved NPXY motifs in the β-integrin cytoplasmic tail. Furthermore, we show that an α-integrin subunit is a BMP target gene, identifying positive feedback between integrin signaling and BMP pathway activity that may contribute to robust cell fate decisions.
Mesh-Begriff(e)	Animals ; Bone Morphogenetic Proteins/physiology ; Cell Line ; Collagen Type IV/genetics ; Collagen Type IV/metabolism ; Drosophila Proteins/physiology ; Drosophila melanogaster/embryology ; Drosophila melanogaster/metabolism ; Embryo, Nonmammalian/metabolism ; Embryonic Development ; Feedback, Physiological ; Gene Expression ; Gene Expression Regulation, Developmental ; Integrins/physiology ; Signal Transduction
Chemische Substanzen	Bone Morphogenetic Proteins ; Collagen Type IV ; Drosophila Proteins ; Integrins
Sprache	Englisch
Erscheinungsdatum	2015-09-08
Erscheinungsland	United States
Dokumenttyp	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2649101-1
ISSN	2211-1247 ; 2211-1247
ISSN (online)	2211-1247
ISSN	2211-1247
DOI	10.1016/j.celrep.2015.08.012
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Pupil dilation reflects the authenticity of received nonverbal vocalizations.

Cosme, Gonçalo / Rosa, Pedro J / Lima, César F / Tavares, Vânia / Scott, Sophie / Chen, Sinead / Wilcockson, Thomas D W / Crawford, Trevor J / Prata, Diana

Scientific reports

2021 Band 11, Heft 1, Seite(n) 3733

Abstract: The ability to infer the authenticity of other's emotional expressions is a social cognitive process taking place in all human interactions. Although the neurocognitive correlates of authenticity recognition have been probed, its potential recruitment of ...

Abstract	The ability to infer the authenticity of other's emotional expressions is a social cognitive process taking place in all human interactions. Although the neurocognitive correlates of authenticity recognition have been probed, its potential recruitment of the peripheral autonomic nervous system is not known. In this work, we asked participants to rate the authenticity of authentic and acted laughs and cries, while simultaneously recording their pupil size, taken as proxy of cognitive effort and arousal. We report, for the first time, that acted laughs elicited higher pupil dilation than authentic ones and, reversely, authentic cries elicited higher pupil dilation than acted ones. We tentatively suggest the lack of authenticity in others' laughs elicits increased pupil dilation through demanding higher cognitive effort; and that, reversely, authenticity in cries increases pupil dilation, through eliciting higher emotional arousal. We also show authentic vocalizations and laughs (i.e. main effects of authenticity and emotion) to be perceived as more authentic, arousing and contagious than acted vocalizations and cries, respectively. In conclusion, we show new evidence that the recognition of emotional authenticity can be manifested at the level of the autonomic nervous system in humans. Notwithstanding, given its novelty, further independent research is warranted to ascertain its psychological meaning.
Sprache	Englisch
Erscheinungsdatum	2021-02-12
Erscheinungsland	England
Dokumenttyp	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-021-83070-x
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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