Artikel ; Online: Seminal plasma proteins of Atlantic halibut (Hippoglossus hippoglossus L.).
Fish physiology and biochemistry
2008 Band 34, Heft 4, Seite(n) 349–355
Abstract: ... 367.4 U l(-l). A high correlation between anti-proteolytic activity and protein concentration (r = 0 ...
Abstract | Protein content and properties in the seminal plasma of Atlantic halibut (Hippoglossus hippoglossus) were assayed using spectrophotometric and electrophoretic methods. The protein concentration ranged from 6.4 +/- 3.1 to 19.4 +/- 3.4 mg ml(-1) and anti-proteolytic activity from 585.2 +/- 104.6 to 2912.4 +/- 367.4 U l(-l). A high correlation between anti-proteolytic activity and protein concentration (r = 0.95), and between sperm concentration and osmolality was found (r = 0.92). There was a significant decrease in anti-proteolytic activity from the first to the second sampling, but not in protein concentration. Anti-proteolytic activity and protein concentration were significantly affected by variations in individual males. Electrophoresis revealed four anti-proteolytic bands and individual differences in bands of proteolytic activity, which were subsequently characterized as metalloproteases and serine proteases. |
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Mesh-Begriff(e) | Animals ; Benzamidines/pharmacology ; Calcium Chloride/pharmacology ; Chelating Agents/pharmacology ; Edetic Acid/pharmacology ; Flounder/metabolism ; Flounder/physiology ; Gelatinases/metabolism ; Male ; Osmolar Concentration ; Semen/drug effects ; Semen/enzymology ; Semen/metabolism ; Seminal Plasma Proteins/metabolism ; Serine Proteinase Inhibitors/pharmacology ; Sperm Count |
Chemische Substanzen | Benzamidines ; Chelating Agents ; Seminal Plasma Proteins ; Serine Proteinase Inhibitors ; Edetic Acid (9G34HU7RV0) ; Gelatinases (EC 3.4.24.-) ; benzamidine (KUE3ZY3J1F) ; Calcium Chloride (M4I0D6VV5M) |
Sprache | Englisch |
Erscheinungsdatum | 2008-12 |
Erscheinungsland | Netherlands |
Dokumenttyp | Journal Article ; Research Support, Non-U.S. Gov't |
ZDB-ID | 292907-7 |
ISSN | 1573-5168 ; 0920-1742 |
ISSN (online) | 1573-5168 |
ISSN | 0920-1742 |
DOI | 10.1007/s10695-007-9194-x |
Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
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