Artikel ; Online: Nucleation seed size determines amyloid clearance and establishes a barrier to prion appearance in yeast.
Nature structural & molecular biology
2020 Band 27, Heft 6, Seite(n) 540–549
Abstract: Amyloid appearance is a rare event that is promoted in the presence of other aggregated proteins. These aggregates were thought to act by templating the formation of an assembly-competent nucleation seed, but we find an unanticipated role for them in ... ...
Abstract | Amyloid appearance is a rare event that is promoted in the presence of other aggregated proteins. These aggregates were thought to act by templating the formation of an assembly-competent nucleation seed, but we find an unanticipated role for them in enhancing the persistence of amyloid after it arises. Specifically, Saccharomyces cerevisiae Rnq1 amyloid reduces chaperone-mediated disassembly of Sup35 amyloid, promoting its persistence in yeast. Mathematical modeling and corresponding in vivo experiments link amyloid persistence to the conformationally defined size of the Sup35 nucleation seed and suggest that amyloid is actively cleared by disassembly below this threshold to suppress appearance of the [PSI |
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Mesh-Begriff(e) | Amyloid/chemistry ; Amyloid/metabolism ; Cycloheximide/pharmacology ; Heat-Shock Proteins/chemistry ; Heat-Shock Proteins/metabolism ; Peptide Termination Factors/chemistry ; Peptide Termination Factors/genetics ; Peptide Termination Factors/metabolism ; Prions/chemistry ; Prions/metabolism ; Saccharomyces cerevisiae/drug effects ; Saccharomyces cerevisiae/metabolism ; Saccharomyces cerevisiae Proteins/chemistry ; Saccharomyces cerevisiae Proteins/genetics ; Saccharomyces cerevisiae Proteins/metabolism |
Chemische Substanzen | Amyloid ; Heat-Shock Proteins ; Peptide Termination Factors ; Prions ; RNQ1 protein, S cerevisiae ; SUP35 protein, S cerevisiae ; Saccharomyces cerevisiae Proteins ; HsP104 protein, S cerevisiae (143012-44-6) ; Cycloheximide (98600C0908) |
Sprache | Englisch |
Erscheinungsdatum | 2020-05-04 |
Erscheinungsland | United States |
Dokumenttyp | Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S. |
ZDB-ID | 2126708-X |
ISSN | 1545-9985 ; 1545-9993 |
ISSN (online) | 1545-9985 |
ISSN | 1545-9993 |
DOI | 10.1038/s41594-020-0416-6 |
Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
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