Artikel: Analysis of Metabolism in Dormant Spores of Bacillus Species by ³¹P Nuclear Magnetic Resonance Analysis of Low-Molecular-Weight Compounds
Journal of bacteriology. 2015 Mar. 1, v. 197, no. 5
2015
Abstract: ... and dormant spore metabolism was monitored by ³¹P nuclear magnetic resonance (NMR) analysis ...
Abstract | This work was undertaken to obtain information on levels of metabolism in dormant spores of Bacillus species incubated for weeks at physiological temperatures. Spores of Bacillus megaterium and Bacillus subtilis strains were harvested shortly after release from sporangia and incubated under various conditions, and dormant spore metabolism was monitored by ³¹P nuclear magnetic resonance (NMR) analysis of molecules including 3-phosphoglyceric acid (3PGA) and ribonucleotides. Incubation for up to 30 days at 4, 37, or 50°C in water, at 37 or 50°C in buffer to raise the spore core pH from ∼ 6.3 to 7.8, or at 4°C in spent sporulation medium caused no significant changes in ribonucleotide or 3PGA levels. Stage I germinated spores of Bacillus megaterium that had slightly increased core water content and a core pH of 7.8 also did not degrade 3PGA and accumulated no ribonucleotides, including ATP, during incubation for 8 days at 37°C in buffered saline. In contrast, spores incubated for up to 30 days at 37 or 50°C in spent sporulation medium degraded significant amounts of 3PGA and accumulated ribonucleotides, indicative of RNA degradation, and these processes were increased in B. megaterium spores with a core pH of ∼7.8. However, no ATP was accumulated in these spores. These data indicate that spores of Bacillus species stored in water or buffer at low or high temperatures exhibited minimal, if any, metabolism of endogenous compounds, even when the spore core pH was 7.8 and core water content was increased somewhat. However, there was some metabolism in spores stored in spent sporulation medium. |
---|---|
Schlagwörter | 3-phosphoglyceric acid ; Bacillus megaterium ; Bacillus subtilis ; RNA ; adenosine triphosphate ; metabolism ; nuclear magnetic resonance spectroscopy ; pH ; ribonucleotides ; sporangia ; spores ; sporulation ; temperature ; water content |
Sprache | Englisch |
Erscheinungsverlauf | 2015-0301 |
Umfang | p. 992-1001. |
Erscheinungsort | American Society for Microbiology |
Dokumenttyp | Artikel |
ZDB-ID | 2968-3 |
ISSN | 1098-5530 ; 0021-9193 |
ISSN (online) | 1098-5530 |
ISSN | 0021-9193 |
DOI | 10.1128/JB.02520-14 |
Datenquelle | NAL Katalog (AGRICOLA) |
Zusatzmaterialien
Kategorien
Verfügbar in ZB MED Bonn
Z 4' 62/105: Hefte anzeigen | ||||
Z 487: Hefte anzeigen |
Über subito bestellen
Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.
Fernleihe an ZB MED
Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.