Artikel ; Online: Cystatin E/M Suppresses Tumor Cell Growth through Cytoplasmic Retention of NF-κB
Molecular and Cellular Biology. 2016 June 1, v. 36, no. 12 p.1776-1792
2016
Abstract: We and others have shown that the cystatin E/M gene is inactivated in primary human tumors, pointing to its role as a tumor suppressor gene. However, the molecular mechanism of tumor suppression is not yet understood. Using plasmid-directed cystatin E/M ... ...
Abstract | We and others have shown that the cystatin E/M gene is inactivated in primary human tumors, pointing to its role as a tumor suppressor gene. However, the molecular mechanism of tumor suppression is not yet understood. Using plasmid-directed cystatin E/M gene overexpression, a lentivirus-mediated tetracycline-inducible vector system, and human papillomavirus 16 (HPV 16) E6 and E7 gene-immortalized normal human epidermal keratinocytes, we demonstrated intracellular and non-cell-autonomous apoptotic growth inhibition of tumor cell lines and that growth inhibition is associated with cytoplasmic retention of NF-κB. We further demonstrated decreased phosphorylation of IκB kinase (IKKβ) and IκBα in the presence of tumor necrosis factor alpha (TNF-α), confirming the role of cystatin E/M in the regulation of the NF-κB signaling pathway. Growth suppression of nude mouse xenograft tumors carrying a tetracycline-inducible vector system was observed with the addition of doxycycline in drinking water, confirming that the cystatin E/M gene is a tumor suppressor gene. Finally, immunohistochemical analyses of cervical carcinoma in situ and primary tumors have shown a statistically significant inverse relationship between the expression of cystatin E/M and cathepsin L and a direct relationship between the loss of cystatin E/M expression and nuclear expression of NF-κB. We therefore propose that the cystatin E/M suppressor gene plays an important role in the regulation of NF-κB. |
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Schlagwörter | Human papillomavirus type 16 ; apoptosis ; cathepsin L ; cell growth ; doxycycline ; gene overexpression ; growth retardation ; humans ; immunohistochemistry ; keratinocytes ; mice ; neoplasm cells ; phosphorylation ; suppressor genes ; tumor necrosis factor-alpha ; tumor suppressor genes ; uterine cervical neoplasms ; xenotransplantation |
Sprache | Englisch |
Erscheinungsverlauf | 2016-0601 |
Umfang | p. 1776-1792. |
Erscheinungsort | Taylor & Francis |
Dokumenttyp | Artikel ; Online |
ZDB-ID | 779397-2 |
ISSN | 1098-5549 ; 0270-7306 |
ISSN (online) | 1098-5549 |
ISSN | 0270-7306 |
DOI | 10.1128/MCB.00878-15 |
Datenquelle | NAL Katalog (AGRICOLA) |
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