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  1. Artikel ; Online: Chronic Jetlag Alters the Landscape of the Pancreatic Lipidome.

    Schwartz, Patrick B / Barrett-Wilt, Gregory A / Ronnekleiv-Kelly, Sean M

    Pancreas

    2022  Band 51, Heft 1, Seite(n) 80–89

    Abstract: Objectives: The innate biologic clock plays a significant role in lipid metabolism, including the peripheral clock in the pancreas. However, an evaluation of the downstream lipids in the pancreatic lipidome is lacking. We sought to understand the ... ...

    Abstract Objectives: The innate biologic clock plays a significant role in lipid metabolism, including the peripheral clock in the pancreas. However, an evaluation of the downstream lipids in the pancreatic lipidome is lacking. We sought to understand the diurnal variations of lipids within the pancreatic lipidome.
    Methods: At 4 weeks of age, C57Bl/6J mice were subjected to either normal lighting conditions or a chronic jetlag (CJ) condition known to mimic chronic shiftwork in humans. At 9 months, mice were serially killed at 4-hour intervals for 24 hours. The pancreas was removed and subjected to untargeted liquid chromatography-mass spectrometry to examine the pancreatic lipidome.
    Results: A total of 4.7% of the pancreatic lipidome was rhythmically expressed, which increased to 12.9% after CJ. After CJ, there was a 4.58-hour shift in the timing of peak 24-hour lipid expression. Chronic jetlag also led to the enrichment of diacylglycerols and triglycerides, while promoting a decrease in lysophosphatidylcholines and 44-carbon acyl chain lipids.
    Conclusions: The pancreatic lipidome exhibits diurnal rhythmicity across a broad number of lipid classes. Chronic jetlag led to alterations in lipid composition that mirrored other metabolically active organs. Several of the reported changes may link altered sleep-wake cycles with known circadian disruption-induced pancreatic diseases.
    Mesh-Begriff(e) Animals ; Circadian Rhythm ; Humans ; Lipidomics ; Mice, Inbred C57BL ; Pancreatic Hormones/metabolism ; Sleep Deprivation/metabolism ; Mice
    Chemische Substanzen Pancreatic Hormones
    Sprache Englisch
    Erscheinungsdatum 2022-02-23
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 632831-3
    ISSN 1536-4828 ; 0885-3177
    ISSN (online) 1536-4828
    ISSN 0885-3177
    DOI 10.1097/MPA.0000000000001962
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel ; Online: Effect of twice-daily oral administration of a chondroitin sulfate-containing supplement on urine chondroitin sulfate concentrations in dogs.

    Wood, Michael W / Barrett-Wilt, Gregory A

    American journal of veterinary research

    2020  Band 80, Heft 8, Seite(n) 799–805

    Abstract: Objective: To quantify the magnitude and duration of changes in urine chondroitin sulfate concentration (uCS) as a result of oral administration of a chondroitin sulfate-containing supplement in dogs.: Animals: 8 healthy privately owned dogs.: ... ...

    Abstract Objective: To quantify the magnitude and duration of changes in urine chondroitin sulfate concentration (uCS) as a result of oral administration of a chondroitin sulfate-containing supplement in dogs.
    Animals: 8 healthy privately owned dogs.
    Procedures: A urine sample was collected from each dog via cystocentesis on day 1; free-catch midstream urine samples were collected once daily on days 2 through 5. Pretreatment uCS was established from those samples. Each dog then received a chondroitin sulfate-containing supplement (20 to 30 mg/kg, PO, q 12 h) for 8 days (on days 7 through 14). Urine samples were collected on days 8 through 12 and day 15. For each sample, uCS was quantified by liquid chromatography-tandem mass spectrometry. Variable urine concentration was accounted for by dividing the uCS by urine creatinine concentration (uCrea) to determine the uCS:uCrea ratio. Pretreatment uCS:uCrea ratios were compared with treatment uCS:uCrea ratios to calculate the fold change in uCS after supplement administration.
    Results: Among the study dogs, oral administration of the chondroitin sulfate-containing supplement resulted in a 1.9-fold increase in the median uCS:uCrea ratio. Data obtained on days 8 through 12 and day 15 indicated that the daily increase in uCS remained consistent and was not additive.
    Conclusions and clinical relevance: Results indicated that oral administration of supplemental chondroitin sulfate to dogs modestly increased uCS within 24 hours; however, subsequent supplement administration did not have an additive effect. A potential therapeutic benefit of persistently increased uCS in preventing recurrent urinary tract infections in dogs warrants investigation.
    Mesh-Begriff(e) Administration, Oral ; Animals ; Chondroitin Sulfates/administration & dosage ; Chondroitin Sulfates/urine ; Chromatography, Liquid/veterinary ; Dietary Supplements ; Dogs/urine ; Drug Administration Schedule ; Female ; Male ; Prospective Studies ; Tandem Mass Spectrometry/veterinary ; Urinalysis/veterinary
    Chemische Substanzen Chondroitin Sulfates (9007-28-7)
    Sprache Englisch
    Erscheinungsdatum 2020-02-05
    Erscheinungsland United States
    Dokumenttyp Clinical Trial, Veterinary ; Journal Article
    ZDB-ID 390796-x
    ISSN 1943-5681 ; 0002-9645
    ISSN (online) 1943-5681
    ISSN 0002-9645
    DOI 10.2460/ajvr.80.8.799
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel: Effect of twice-daily oral administration of a chondroitin sulfate–containing supplement on urine chondroitin sulfate concentrations in dogs

    Wood, Michael W / Barrett-Wilt, Gregory A

    American journal of veterinary research. 2019 Aug., v. 80, no. 8

    2019  

    Abstract: OBJECTIVE To quantify the magnitude and duration of changes in urine chondroitin sulfate concentration (uCS) as a result of oral administration of a chondroitin sulfate–containing supplement in dogs. ANIMALS 8 healthy privately owned dogs. PROCEDURES A ... ...

    Abstract OBJECTIVE To quantify the magnitude and duration of changes in urine chondroitin sulfate concentration (uCS) as a result of oral administration of a chondroitin sulfate–containing supplement in dogs. ANIMALS 8 healthy privately owned dogs. PROCEDURES A urine sample was collected from each dog via cystocentesis on day 1; free-catch midstream urine samples were collected once daily on days 2 through 5. Pretreatment uCS was established from those samples. Each dog then received a chondroitin sulfate–containing supplement (20 to 30 mg/kg, PO, q 12 h) for 8 days (on days 7 through 14). Urine samples were collected on days 8 through 12 and day 15. For each sample, uCS was quantified by liquid chromatography–tandem mass spectrometry. Variable urine concentration was accounted for by dividing the uCS by urine creatinine concentration (uCrea) to determine the uCS:uCrea ratio. Pretreatment uCS:uCrea ratios were compared with treatment uCS:uCrea ratios to calculate the fold change in uCS after supplement administration. RESULTS Among the study dogs, oral administration of the chondroitin sulfate–containing supplement resulted in a 1.9-fold increase in the median uCS:uCrea ratio. Data obtained on days 8 through 12 and day 15 indicated that the daily increase in uCS remained consistent and was not additive. CONCLUSIONS AND CLINICAL RELEVANCE Results indicated that oral administration of supplemental chondroitin sulfate to dogs modestly increased uCS within 24 hours; however, subsequent supplement administration did not have an additive effect. A potential therapeutic benefit of persistently increased uCS in preventing recurrent urinary tract infections in dogs warrants investigation.
    Schlagwörter additive effect ; chondroitin sulfate ; creatinine ; dog diseases ; dogs ; liquid chromatography ; oral administration ; tandem mass spectrometry ; urinary tract diseases ; urine ; veterinary medicine
    Sprache Englisch
    Erscheinungsverlauf 2019-08
    Umfang p. 799-805.
    Erscheinungsort American Veterinary Medical Association
    Dokumenttyp Artikel
    ZDB-ID 390796-x
    ISSN 1943-5681 ; 0002-9645
    ISSN (online) 1943-5681
    ISSN 0002-9645
    DOI 10.2460/ajvr.80.8.799
    Datenquelle NAL Katalog (AGRICOLA)

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  4. Artikel ; Online: Assessment of Truck-Mounted Area-Wide S-methoprene Applications to Manage West Nile Virus Vector Species in the Suburbs of Chicago, IL, USA.

    Johnson, Haley E / Clifton, Mark / Harbison, Justin E / Erkapic, Anastazia / Barrett-Wilt, Gregory A / Paskewitz, Susan / Bartholomay, Lyric

    Journal of medical entomology

    2022  Band 60, Heft 2, Seite(n) 384–391

    Abstract: West Nile virus remains the leading cause of arboviral neuroinvasive disease in the United States, despite extensive efforts to control the mosquito vectors involved in transmission. In this study, we evaluated the effectiveness of Altosid SR-20 (active ... ...

    Abstract West Nile virus remains the leading cause of arboviral neuroinvasive disease in the United States, despite extensive efforts to control the mosquito vectors involved in transmission. In this study, we evaluated the effectiveness of Altosid SR-20 (active ingredient, S-methoprene 20%) larvicide applications using truck-mounted ultra-low volume (ULV) dispersal equipment to target Culex pipiens Linnaeus (Diptera: Culicidae) and Cx. restuans (Theobald)larvae. A combination of emergence bioassays, open-field measurements of deposited S-methoprene and spray distribution using gas chromatography-mass spectrometry, and assessments of adult Culex spp. populations in response to applications were conducted over the summer of 2020 within the North Shore Mosquito Abatement District (IL, USA). Open-field applications revealed that dispersed Altosid SR-20 using ULV equipment was effective (75% emergence inhibition in susceptible lab strain Cx. pipiens larvae) up to 53 m. In suburban neighborhood applications, we found that S-methoprene deposition and larval emergence inhibition (EI) in front yards did not differ significantly from backyards. An overall EI of 46% and 28% were observed for laboratory strain Cx. pipiens and wild Cx. restuans larvae respectively, and both had an EI significantly higher than the untreated control group. The EI of exposed wild Cx. pipiens larvae did not differ from the untreated controls, suggesting an increased tolerance to S-methoprene. No difference in abundance of gravid or host-seeking adult Culex spp. post-application was detected between treated and untreated sites. These results document the ability of area-wide application to distribute S-methoprene, but this strategy will need further modifications and evaluation for Culex spp. management.
    Mesh-Begriff(e) Animals ; West Nile virus ; Methoprene ; Chicago ; Mosquito Vectors ; Seasons ; Culex/physiology ; Larva ; West Nile Fever/prevention & control
    Chemische Substanzen Methoprene (8B830OJ2UX)
    Sprache Englisch
    Erscheinungsdatum 2022-11-05
    Erscheinungsland England
    Dokumenttyp Journal Article ; Research Support, U.S. Gov't, P.H.S.
    ZDB-ID 410635-0
    ISSN 1938-2928 ; 0022-2585
    ISSN (online) 1938-2928
    ISSN 0022-2585
    DOI 10.1093/jme/tjac170
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  5. Artikel ; Online: Bacteria: A novel source for potent mosquito feeding-deterrents.

    Kajla, Mayur K / Barrett-Wilt, Gregory A / Paskewitz, Susan M

    Science advances

    2019  Band 5, Heft 1, Seite(n) eaau6141

    Abstract: Antibiotic and insecticidal bioactivities of the extracellular secondary metabolites produced by entomopathogenic bacteria belonging to ... ...

    Abstract Antibiotic and insecticidal bioactivities of the extracellular secondary metabolites produced by entomopathogenic bacteria belonging to genus
    Mesh-Begriff(e) Aedes/drug effects ; Aedes/physiology ; Animals ; Anopheles/drug effects ; Anopheles/physiology ; Culex/drug effects ; Culex/physiology ; DEET/pharmacology ; Dose-Response Relationship, Drug ; Drug Evaluation, Preclinical/instrumentation ; Drug Evaluation, Preclinical/methods ; Feeding Behavior/drug effects ; Female ; Insect Repellents/administration & dosage ; Insect Repellents/chemistry ; Insect Repellents/pharmacology ; Oligopeptides/chemistry ; Piperidines/pharmacology ; Polyamines/chemistry ; Xenorhabdus/chemistry
    Chemische Substanzen Insect Repellents ; Oligopeptides ; Piperidines ; Polyamines ; fabclavine Ia ; DEET (134-62-3) ; picaridin (N51GQX0837)
    Sprache Englisch
    Erscheinungsdatum 2019-01-16
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2810933-8
    ISSN 2375-2548 ; 2375-2548
    ISSN (online) 2375-2548
    ISSN 2375-2548
    DOI 10.1126/sciadv.aau6141
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  6. Artikel ; Online: Dietary adaptation to high starch involves increased relative abundance of sucrase-isomaltase and its mRNA in nestling house sparrows.

    Brun, Antonio / Magallanes, Melisa E / Barrett-Wilt, Gregory A / Karasov, William H / Caviedes-Vidal, Enrique

    American journal of physiology. Regulatory, integrative and comparative physiology

    2020  Band 320, Heft 2, Seite(n) R195–R202

    Abstract: Dietary flexibility in digestive enzyme activity is widespread in vertebrates but mechanisms are poorly understood. When laboratory rats are switched to a higher carbohydrate diet, the activities of the apical intestinal α-glucosidases (AGs) increase ... ...

    Abstract Dietary flexibility in digestive enzyme activity is widespread in vertebrates but mechanisms are poorly understood. When laboratory rats are switched to a higher carbohydrate diet, the activities of the apical intestinal α-glucosidases (AGs) increase within 6-12 h, mainly by rapid increase in enzyme transcription, followed by rapid translation and translocation to the intestine's apical, brush-border membrane (BBM). We performed the first unified study of the overall process in birds, relying on activity, proteomic, and transcriptomic data from the same animals. Our avian model was nestling house sparrows (
    Mesh-Begriff(e) Adaptation, Physiological/drug effects ; Aging ; Animal Feed ; Animals ; Diet/veterinary ; Dietary Carbohydrates/pharmacology ; Gene Expression Regulation, Enzymologic/drug effects ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Sparrows/physiology ; Starch/administration & dosage ; Starch/pharmacology ; Sucrase-Isomaltase Complex/genetics ; Sucrase-Isomaltase Complex/metabolism
    Chemische Substanzen Dietary Carbohydrates ; RNA, Messenger ; Starch (9005-25-8) ; Sucrase-Isomaltase Complex (EC 3.2.1.-)
    Sprache Englisch
    Erscheinungsdatum 2020-11-11
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 603839-6
    ISSN 1522-1490 ; 0363-6119
    ISSN (online) 1522-1490
    ISSN 0363-6119
    DOI 10.1152/ajpregu.00181.2020
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  7. Artikel ; Online: Identification of Molecular Targets of Dietary Grape-Mediated Chemoprevention of Ultraviolet B Skin Carcinogenesis: A Comparative Quantitative Proteomics Analysis.

    Mintie, Charlotte A / Singh, Chandra K / Ndiaye, Mary A / Barrett-Wilt, Gregory A / Ahmad, Nihal

    Journal of proteome research

    2019  Band 18, Heft 10, Seite(n) 3741–3751

    Abstract: We recently showed that dietary grape powder (GP) imparts considerable protection against ultraviolet B (UVB)-mediated skin carcinogenesis in SKH-1 mice. To determine molecular mechanisms of this response, we employed tandem mass tag (TMT) quantitative ... ...

    Abstract We recently showed that dietary grape powder (GP) imparts considerable protection against ultraviolet B (UVB)-mediated skin carcinogenesis in SKH-1 mice. To determine molecular mechanisms of this response, we employed tandem mass tag (TMT) quantitative global proteomics approach on skin tumors from mice exposed to 180 mJ/cm
    Mesh-Begriff(e) Animals ; Chemoprevention/methods ; Diet ; Drug Delivery Systems ; Mice ; Oxidative Stress ; Proteolysis ; Proteomics/methods ; Skin Neoplasms/etiology ; Skin Neoplasms/prevention & control ; Tandem Mass Spectrometry ; Ubiquitination ; Ultraviolet Rays/adverse effects ; Vitis/chemistry
    Sprache Englisch
    Erscheinungsdatum 2019-09-23
    Erscheinungsland United States
    Dokumenttyp Comparative Study ; Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 2078618-9
    ISSN 1535-3907 ; 1535-3893
    ISSN (online) 1535-3907
    ISSN 1535-3893
    DOI 10.1021/acs.jproteome.9b00442
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  8. Artikel ; Online: A Fast and Accurate Method to Identify and Quantify Enzymes in Brush-Border Membranes: In Situ Hydrolysis Followed by Nano LC-MS/MS.

    Brun, Antonio / Magallanes, Melisa E / Martínez Del Rio, Carlos / Barrett-Wilt, Gregory A / Karasov, William H / Caviedes-Vidal, Enrique

    Methods and protocols

    2020  Band 3, Heft 1

    Abstract: A simple method for the identification of brush-border membrane α-glucosidases is described. The proteins were first solubilized and separated in a gel under native, non-denaturing, conditions. The gel was then incubated in substrate solutions (maltose ... ...

    Abstract A simple method for the identification of brush-border membrane α-glucosidases is described. The proteins were first solubilized and separated in a gel under native, non-denaturing, conditions. The gel was then incubated in substrate solutions (maltose or sucrose), and the product (glucose) exposed in situ by the oxidation of o-dianisidine, which yields a brown-orange color. Nano-liquid chromatography coupled to mass spectrometry analyses of proteins (nano LC-MS/MS) present in the colored bands excised from the gels, was used to confirm the presence of the enzymes. The stain is inexpensive and the procedure permits testing several substrates in the same gel. Once enzymes are identified, their abundance, relative to that of other proteins in the brush border, can be semi-quantified using nano LC-MS/MS.
    Sprache Englisch
    Erscheinungsdatum 2020-02-10
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ISSN 2409-9279
    ISSN (online) 2409-9279
    DOI 10.3390/mps3010015
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  9. Artikel ; Online: Co-culture with mouse embryonic fibroblasts improves maintenance of metabolic function of human small hepatocyte progenitor cells.

    Sengupta, Srikumar / Johnson, Brian / Seirup, Morten / Ardalani, Hamisha / Duffin, Bret / Barrett-Wilt, Gregory A / Stewart, Ron / Thomson, James A

    Current research in toxicology

    2020  Band 1, Seite(n) 70–84

    Abstract: Derivation and culture of small hepatocyte progenitor cells (SHPCs) capable of ... ...

    Abstract Derivation and culture of small hepatocyte progenitor cells (SHPCs) capable of proliferating
    Sprache Englisch
    Erscheinungsdatum 2020-08-26
    Dokumenttyp Journal Article
    ISSN 2666-027X
    ISSN (online) 2666-027X
    DOI 10.1016/j.crtox.2020.08.001
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  10. Artikel ; Online: Aronia Berry Supplementation Mitigates Inflammation in T Cell Transfer-Induced Colitis by Decreasing Oxidative Stress.

    Pei, Ruisong / Liu, Jiyuan / Martin, Derek A / Valdez, Jonathan C / Jeffery, Justin / Barrett-Wilt, Gregory A / Liu, Zhenhua / Bolling, Bradley W

    Nutrients

    2019  Band 11, Heft 6

    Abstract: Oxidative stress is involved in the pathogenesis and progression of inflammatory bowel disease. Consumption of aronia berry inhibits T cell transfer colitis, but the antioxidant mechanisms pertinent to immune function are unclear. We hypothesized that ... ...

    Abstract Oxidative stress is involved in the pathogenesis and progression of inflammatory bowel disease. Consumption of aronia berry inhibits T cell transfer colitis, but the antioxidant mechanisms pertinent to immune function are unclear. We hypothesized that aronia berry consumption could inhibit inflammation by modulating the antioxidant function of immunocytes and gastrointestinal tissues. Colitis was induced in recombinase activating gene-1 deficient (
    Mesh-Begriff(e) Animals ; Antioxidants/pharmacology ; CD4-Positive T-Lymphocytes/drug effects ; Colitis/chemically induced ; Colitis/immunology ; Dietary Supplements ; Disease Models, Animal ; Fruit ; Inflammation ; Interferon-gamma/metabolism ; Intestines/immunology ; Mice ; Oxidative Stress/drug effects ; Photinia ; Tumor Necrosis Factor-alpha/metabolism
    Chemische Substanzen Antioxidants ; Tumor Necrosis Factor-alpha ; Interferon-gamma (82115-62-6)
    Sprache Englisch
    Erscheinungsdatum 2019-06-12
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2518386-2
    ISSN 2072-6643 ; 2072-6643
    ISSN (online) 2072-6643
    ISSN 2072-6643
    DOI 10.3390/nu11061316
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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